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Dispenser set needed for the Malonaldehyde Test Kit. Includes one dispenser for each reagent (A, B, and Preparation Reagent) and one reagent holding rack.

Dispenser set needed for the Percent Fat Test Kit. Includes one dispenser for each reagent (A and Preparation Reagent).

Dispenser set needed for the Peroxide Test Kit. Includes one dispenser for each reagent (A, B, C, and Preparation Reagent) and one reagent holding rack.

Dispenser set needed for the Total Glycerin Test Kit. Includes one dispenser for each reagent (A and Preparation Reagent).

The DynaMag-2 magnet combines a strong magnetic attraction for efficient separations in standard microcentrifuge tubes with a flexible and smart ergonomic design.

• • Optimal working volume: 10–2000µL

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• • Holds up to 16 standard 1.5–2mL microcentrifuge tubes in numbered spaces

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• • Good control and visibility of sample

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• • Top rack can be quickly removed from magnet in base, ready for resuspension, vortexing, rotation, or manual sample shaking

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• • Magnet can be used with all types of Dynabeads for efficient isolation of proteins and nucleic acids

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• • Sample rack can be easily removed from base magnet for resuspension, vortexing, rotation or manual sample shaking

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• • Center pin in rack ensures equal vortexing of all tubes

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• • Rack can also be removed and used to store tubes

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• • 16 numbered sample spaces make magnet for parallel handling of multiple samples

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• • With eight tube positions on each side of rack, easily scale protocols to accommodate 96-well plate reactions

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This magnet is not compatible with the MX4 Mixer. The removable sample rack is also available as a separate item. Purchasing an additional sample rack minimizes the need to move tubes between racks. The rack can be placed on the bench, in the refrigerator, or in the incubator.nnCell Activation and Expansion, Cell Analysis, Cell Isolation, DNA and RNA Purification and Analysis, DNA Extraction, Immunoprecipitation, Magnets for Cell Isolation, Protein Sample Preparation and Protein Purification, Proteins, Expression, Isolation and Analysis, Sequence-Specific DNA or RNA Purification, Sequencing Reaction Purification.

The E-Gel™ 1Kb Plus DNA ladder is composed of 20 highly purified, double-stranded DNA bands spanning 100 base pairs to 12,000 base pairs. Ladder has 12 evenly spaced bands ranging from 1Kb to 12Kb, quick orientation band at 1,650 base pairs that forms distinct doublet with 2Kb band, and seven bands of round sizes below 1Kb. The E-Gel 1Kb Plus DNA Ladder is specifically formulated with minimal salts to perform exceptionally well on E-Gel™ EX pre-cast agarose gels. Unlike some other DNA ladders, E-Gel 1Kb Plus DNA Ladder has been specially designed to have bands of round, easy-to-remember sizes. Logical band layout, and quick identification doublet, allow quick and easy estimates of band size at glance.

• • Round, easy-to-remember band sizes

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• • Broad size range (100bp–12Kb)

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• • Formulated specifically for E-Gel EX gels

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• • Room temperature stable for easy storage

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• • Single ladder fits most needs

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• • Ready-to-use formulation for E-Gel and E-Gel EX gels

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Agarose Gel Electrophoresis, DNA and RNA Purification and Analysis, Nucleic Acid Gel Electrophoresis and Blotting.

Perform sample transfers between virtually any tube, rack, microplate or horizontal gel box quickly and efficiently with the Thermo Scientific™ E1-ClipTip™ Equalizer Pipette. Adjustable tip spacing allows you to set the distance between tips by simply sliding the scale to expand or contract to the desired labware format.The NEW My Pipette™ Creator app enables programming of E1-ClipTip from the comfort of your PCnn nnInnovative ClipTip interlocking tip interface technology.

• • Tip is locked and sealed in place until ejected — no dropped tips that waste valuable samples

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• • Complete seal to secure the sample volume in each tip for enhanced accuracy and precision

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• • Tips are attached and ejected with a light touch — eliminates the frustration of banging tips on the pipette

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My Pipette Creator to revolutionize the way you pipette with a new era of technology

• • New web-based pipetting app powered by Thermo Fisher Cloud

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• • Enables efficient, centralized programming and sharing of protocols between E1-ClipTip pipettes and colleagues

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• • Offers pre-programmed protocols, to download and share, for many of your favorite reagent kits and pipetting protocols

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• • Connect E1-ClipTip pipette to the app via a Bluetooth** or USB connection.

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• • Accelerate collaboration and reproducibility, all from the comfort of your computer.

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E1-ClipTip pipette to help you enjoy pipetting

• • E1-ClipTip is designed for optimum comfort and ease of use: the electronic tip ejection and index finger pipetting action let your thumb relax during pipetting.

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• • Two operating options: Step-based Matrix or Presets with settings for your most common applications.

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• • The Programs function allows you to store 30 your most common Matrix or Presets protocols with a specific name.

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• • Connect your E1-ClipTip pipette to My Pipette Creator app conveniently via Bluetooth**

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• • Five different user profiles offer customized settings for multiple users and protocols

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• • MyPipette™ function to customize your main menu view by organizing the icons in your preferred order or creating shortcuts for your favorite programs and pipetting functions. You can also protect important settings from unintentional editing with a password.

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• • Service and calibration trackers remind you when the pipette needs to be sent for service

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• • Li-ion battery for long lasting performance and fast recharging. Recharging with a charging stand or a plug, you decide.

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• • 2-year warranty with convenient web registration

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E1-ClipTip™ Equalizer: transferring multiple samples between different labware formats

• • Adjustable tip spacing allows you to set the distance between pipette tips for different types of labware simply by sliding a scale to expand or contract the tip spacing to the desired format:
    • • Microcentrifuge tubes to/from 24,-48,-96-and 384- well microplates

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    • • Test tubes to/from deepwell blocks

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    • • Microplates and tubes to agarose gels

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• • Save up to 90% in time and repetitions compared to use of single channel manual pipettes***

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E1-ClipTip pipette and ClipTip tips for every applicationn

• • Choose your E1-ClipTip, volume range from 0.5 µL to 1250µL:n
    • • Single channels

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    • • Multichannels with 8, 12 or 16 channels in 96 and 384 formats

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    • • Adjustable tip spacing multichannels with 6, 8, 12 channels in 96 and 384 formats

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    Wide selection of ClipTip tips:

    • • ClipTip 96-format tips are available in 20, 50, 200, 300, 1000 and 1250 µl

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    • • ClipTip 384-format tips are available in 12.5, 30 and 125 µl

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    • • Several packaging options: filtered, sterile, non-sterile, racked and reload

    Compatible with Thermo Scientific ClipTip pipette tips.

* E1-ClipTip 12.5 and all 384 E1-ClipTip pipettes utilize snap and seal technology.
** Bluetooth models available in following countries/regions: Andorra, Australia, Austria, Belgium, Bulgaria, Canada, China, Croatia, Cyprus, Czech Republic, Denmark, Estonia, Finland, France, Germany, Greece, Hong Kong, Hungary, Iceland, India, Ireland, Italy, Japan, Korea (Rep. of), Latvia, Liechtenstein, Lithuania, Luxembourg, Malta, Monaco, Netherlands, New Zealand, Norway, Poland, Portugal, Romania, San Marino, Singapore, Slovakia, Slovenia, Spain, Sweden, Switzerland, Taiwan, United Kingdom, United States and Vatican. For other countries catalog numbers without ‘BT’ extension should be used.
*** Compared to a manual single channel pipette filling a 96-well microplate from microcentrifuge tubes. See Thermo Scientific Smart Note: Is there an easier and more efficient way to transfer liquids between various labware formats, than using traditional handheld pipettes? 2014.

Disclaimer to internal research use restriction: Any restrictions on the purchaser to utilize the product(s) for internal research purposes only does not apply to the product(s) on the web pages associated with this statement.

The MiniCuve 8 x 10 cm electrophoresis unit is machined from a solid block of acrylic to eliminate the possibility of leaks or breakage. Buffer volume is only 100 mL. The MiniCuve is delivered complete with lid, gel tray and connecting wires. Numerous different combs are available.

• • Provides all of the benefits of Essential 8 Medium such as minimized variability of components, reliable and robust cultures with a xeno-free, cGMP produced media

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• • Convenient two-component kit (500mL basal medium and 10mL supplement), and when used with vitronectin (VTN-N), provides defined system for feeder-free culture of human PSCs

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• • Flexibility is an added benefit

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Order Info

Approved for shipment at Room Temperature or on Wet Ice

• Supports growth of suspension 293 cultures to densities of over 1.5 x 10^7 cells/mL
• Transfection compatible serum-free suspension culture medium enables transfection efficiencies of greater than 70% using ExpiFectamine™ transfection reagent
• Enables sustained, high level expression of high density transiently transfected cultures, achieving yields of up to 1 gram per liter of recombinant protein
• Supports growth and transfection of 293 cells in culture formats of less than 1mL in multiwell plates to greater than 10L in disposable bioreactors

• • Supports growth of suspension CHO cultures to densities of over 2 x 107 cells per mL, with >95% cell viability

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• • Uses a transfection-compatible, serum-free suspension culture medium that enables high transfection efficiencies of >90% with ExpiFectamine™ CHO transfection reagent

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• • Supports transient transfection of suspension CHO cells at a density of 6 x 106 cells per mL

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• • Facilitates sustained, high-level expression of high-density transiently transfected cultures, achieving yields of up to 3 g per L of recombinant protein

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• • Supports growth and transfection of CHO cells in culture formats from <1 mL in multi-well plates to >10 L in benchtop bioreactors

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• • Does not contain phenol red

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Frame TransDNA

FRONT PANEL FOR TEMPLATE-TAMER

Casting tray for 2 GelTrays. Made of plexiglas, trays incorporates a special designed seal to prevent spillage.

Casting tray for MiniCuve electrophoresis 6 GelTrays. Made of plexiglas, trays incorporates a special designed seal to prevent spillage.

Gel Trays to casting agarose gels for MiniCuve

Gel Trays to casting agarose gels for MiniCuve

• • Mercaptoethanol is not stable in solution. so most protocols require daily supplementation

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• • Concentration of 55mM in Dulbecco’s Phosphate Buffered Saline (DPBS)

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• • Manufactured at a cGMP compliant facility, located in Grand Island, New York, registered with the FDA as a medical device manufacturer and certified to ISO 13485 standards

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GIBCO OpTmizer™ CTS™ T-Cell Expansion SFM has been developed for the growth and expansion of human T lymphocytes. OpTmizer™ CTS™ T-Cell Expansion medium is a complete serum-free, xeno-free 1X medium consisting of OpTmizer™ T-Cell Expansion Basal Medium (1 L bottle) and OpTmizer™ T-Cell Expansion Supplement (26 mL), which are mixed together prior to use.

• • Maintains similar phenotype and function (e.g., cytokine secretion profile) of polyclonally activated and cultured T cells as observed for conventional serum supplemented medium

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• • Consistent performance in supporting T cell expansion

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• • Supports high density T cell culture (e.g., >3×10⁶ CD3+ T cells/mL) in static culture
    
    With OpTmizer™ T-Cell Expansion SFM, we offer both basic and clinical research communities a new tool to enhance their success in growing human T-cells. It is specially formulated to allow for superior cell growth & viability, less variability and more consistent results, and easier transition from research to clinical applications.
    
    Workflow benefits:

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• • Minimizing the need to handle purchasing, storage, qualification of serum and thus reducing variability and costs

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• • More rapid T cell expansion, thus reducing cycle time and improving productivity

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• • Complete serum-free, xeno-free medium, allowing for a seamless transition from research to clinical applications and minimizing regulatory workload
    
    “This reagent is classified as a medical device in several countries. If it is not regulated as a medical device in specific region, it should be used for laboratory use only.”

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Description

• • Suitable for both adherent and suspension culture of mammalian cells, with no adaptation required

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• • Offered as a 200mM L-alanyl-L-glutamine dipeptide in 0.85% NaCl

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• • Compared to L-glutamine: Minimizes toxic ammonia build-up; Improves cell viability and growth; Remains stable across a wide range of temperaturesI

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• • Cells cleave the dipeptide bond to release L-glutamine as needed, preventing waste build-up and maintaining a fresh supply of L-glutamine during long-term cultureI

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• • Cultures with lower levels of ammonia show improved cell viability

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• • In addition, culture conditions are more controlled with the consistent concentration not possible with L-glutamineI

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• • Room-temperature stable and ready to use

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• • Manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK, registered with the FDA as a medical device manufacturer and certified to the ISO 13485 standard

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Cell Culture, Clinical, Clinical and Translational Research, Cytogenetics, Karyotyping, Mammalian Cell Culture

Opti-MEMI medium is also recommended for use with cationic lipid transfection reagents, such as Lipofectamine™ reagent. Opti-MEM I medium can be used with a variety of suspension and adherent mammalian cells, including Sp2, AE-1, CHO, BHK-21, HEK, and primary fibroblasts.

• • Opti-MEM I is modified as follows: with L-glutamine, phenol red

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• • Unique medium that contains insulin, transferrin, hypoxanthine, thymidine, and trace elements

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• • Additional components allow for reduction in serum supplementation by at least 50%

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• • Uses sodium bicarbonate buffer system (2.4g/L), and therefore requires a 5-10% CO₂ environment to maintain physiological pH

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• • Sustainable packaging

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Cell Culture, Mammalian Cell Culture, Plasmid Transfection, RNAi Transfection, Synthetic siRNA Transfection, Transfection, shRNA and miR RNAi Plasmid Transfection.

Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco™ PBS may require pH adjustment at the time of dilution (see protocol for details).

We offer a variety of Gibco™ PBS formulations for a range of cell culture applications.

PBS (phosphate buffered saline) is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. This PBS is manufactured without calcium, magnesium or phenol red. The complete formulation is available.

• • Animal origin-free

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• • Sustainable packaging

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Cell Culture, Mammalian Cell Culture

• • Used to reduce foaming in stirred cultures and reduce cell attachment to glass

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• • Provided at concentration of 10% and effective at working concentration of 0.1%

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• • Manufactured at a cGMP compliant facility, located in Grand Island, New York, registered with the FDA as a medical device manufacturer and certified to ISO 13485 standards

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Cell Culture, Clinical, Clinical and Translational Research, Cytogenetics, Karyotyping, Mammalian Cell Culture

Bacto Casamino Acids is an animal origin (AO) acid hydrolysis of casein. This supplement contains low salt and iron content making it an excellent supplement for media formulations where nitrogen requirements are minimal. This supplement is successfully used in fermentation applications for pneumococcal, diphtheria, pertussis, tetanus, and zoster vaccines. It maintains many of the nutritional benefits of hydrolyzed casein, but is further supplemented with growth factors, cystine, maltose, and an optimum amount of iron and inorganic salts.

Customers have used Bacto Casamino Acids to support the growth of Bordetella, Corynebacterium, Pseudomonas, Haemophilus, and Escherichia species. Additionally, together with Bacto Yeast Extract, technical, customers have used this supplement successfully in the production of animal and human health vaccines.

Bacto Neopeptone is an animal origin (AO) enzymatic digest of bovine (milk) and porcine protein. This nutritional supplement provides vitamins, nucleotides, minerals, and peptides in a variety of sizes to any culture formulation. Customers have used Bacto Neopeptone as a component of culture media in the cultivation of human pathogens, notably Bordetella pertussis and group A streptococci, as well as Borrelia burgdorferi and Clostridium species, to produce vaccines for animal health applications.

Features:
• Enzymatic digest of bovine (milk) and porcine protein
• Animal origin nutritional supplement
• Contains a wide variety of peptide sizes in combination with vitamins, nucleotides, and minerals

Applications of Bacto Neopeptone include:
• Recommended for use in media for detection of fungi
• Reported to provide nutrients for support of spirochetes and protozoa
• Microbial fermentation
• Production of vaccines for animal health applications

Bacto Proteose Peptone No. 2 is member of the Bacto Proteose Peptones, which are enzymatic digests of protein. Studies of peptic digests of animal tissue prepared under varying digestion parameters demonstrated that no single peptone is the most suitable nitrogen source for every microbiological application. These findings led to the development of Bacto Proteose Peptone, Bacto Proteose Peptone No. 2, and Bacto Proteose Peptone No. 3. Bacto Proteose Peptone No. 4 is a spray-dried version of Bacto Proteose Peptone.nn• Bacto Proteose Peptone is used in preparing microbiological culture media and in producing bacterial toxinsn• Bacto Proteose Peptone No. 2 is used in preparing microbiological culture median• Bacto Proteose Peptone No. 3 is used in preparing microbiological and mammalian cell culture median• Bacto Proteose Peptone No. 4 is a spray-dried version of Bacto Proteose Peptone. It offers the same beneficial nutrients as Bacto Proteose Peptone for growth promotion and toxin production in a wide range of fastidious microorganisms.

Bacto Tryptose is a mixed enzymatic hydrolysate with distinctive nutritional properties. The digestive process of tryptose results in assorted peptides of higher molecular weight suitable for long-chain amino acid requirements.

Features:
• Reported as beneficial for cell culture applications
• Animal origin (AO) nutritional supplement

Applications of Bacto Tryptose include:
• Tryptose is very useful for cultivation of streptococci, pneumococci, meningococci,
and other fastidious organisms.
• Tryptose Peptone is often used as a biomass enhancer for recombinant Escherichia coli production

Bacto Yeast Extracts are concentrates of the water-soluble portion of autolyzed Saccharomyces cerevisiae cells. They are derived from primary grown baker’s yeast. These animal origin-free products are suitable for use as multi-functional nutritional supplements in mammalian cell culture, microbial fermentation, and insect cell culture applications. Yeast extract provides essential water-soluble vitamins, amino acids, peptides, and carbohydrates to any medium formulation.

Bacto Yeast Extract is one of the most complete and versatile fermentation supplements available. It is an important ingredient for the microbiological assay of vitamins. Yeast extract is also of value in the assay of antibiotics. B factor, a growth substance necessary for the production of rifampin in a Nocardia species, can be isolated from yeast extract. Bacto Yeast Extract, Technical, was developed to provide products priced for the biotechnology market with acceptable clarity and growth-promoting characteristics.

Related yeast extract products:
• Gibco Yeast Extract—developed to provide a product for the biotechnology/pharmaceutical market with acceptable clarity and growth-promoting characteristics. Media formulations containing yeast extract are specified in standard methods for various applications.
• Difco Yeast Extract UF—ultra-filtered and specifically designed for mammalian cell culture applications. With its low endotoxin level and high content of naturally occurring B vitamins, it can be used effectively to reduce the need for fetal bovine serum. It has an endotoxin level of less than or equal to 500 EU/g.
• Difco Yeast Extract Low-Dusting (LD)—designed to limit the amount of dust generated compared to other spray-dried yeast extracts

• • Collagenase is a protease that cleaves the bond between a neutral amino acid (X) and glycine in the sequence Pro-X-Gly-Pro, which is found with high frequency in collagen

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• • It is unique among proteases in its ability to degrade the triple-helical native collagen fibrils commonly found in connective tissues such as skin, tendon, blood vessels, and bone

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• • Collagenase disaggregation is suitable for the culture of human tumors, mouse kidney, human adult and fetal brain, and many other tissues including epithelium

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• • Collagenase is relatively gentle, dissociates well at physiological temperature and pH, and requires no mechanical agitation or special equipment

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• • Gibco™ Collagenase Type I is isolated from Clostridium histolyticum and packaged as a lyophilized, non-sterile powder for research use in cell or tissue dissociation, and organ perfusions

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• • Compared to other collagenase preparations, Gibco™ Collagenase Type I has average levels of collagenase, caseinase, clostripain, and tryptic activities, and is well-suited for the digestion of fat, adrenal, and liver cells or tissues

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• • Gibco™ Collagenase Type I activity is guaranteed to be greater than 125 units/mg

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• • Store at 2°C to 8°C, protected from light

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Difco Beef Extract is an animal origin peptone derived from an infusion of beef. This nutritional supplement provides a mixture of peptides, individual amino acids, nucleotides, organic acids, minerals, and vitamins. Provided as a paste, customers have used Difco Beef Extract in a variety of microbial fermentation cell lines, including Escherichia coli, Bacillus subtillis, Pasteurella multocida, and Streptococcus species to produce numerous animal health vaccines.

Features:
• An animal origin nutritional supplement
• A mixture of peptides, individual amino acids, nucleotides, organic acids, minerals, and vitamins

Applications of Difco Beef Extract include:
• Use in a variety of microbial fermentation cell lines
• Used to produce numerous animal health vaccines

Difco Tryptone Peptone is an animal origin (AO) pancreatic digest of casein. In addition to providing a unique nutritional profile, it has a higher nitrogen content than our other casein products. Tryptone Peptone supports the growth of many different types of bacteria and fungi, including fastidious organisms and in toxin production. Customers have used this supplement in the development of animal health vaccines, such as Clostridial vaccines, and with human health Botox-like products produced from Clostridium botulinum.

Features:
• Animal origin, pancreatic digest of casein
• Primary nitrogen source in trypticase soy broth and agar
• Referenced in Official Methods of Analysis of AOAC International
• Supports the growth of many different types of bacteria and fungi

Sera for cell culture with robust cell lines
• Good value for basic research
• Endotoxin level: = 20 EU/mL (levels routinely = 10 EU/mL)
• Hemoglobin level: = 25 mg/dL
• Origin: Refer to CoA for specific origin (US, Canada, Mexico, Brazil, Australia, New Zealand)n

Gibco serum delivers:
• ISO 13485-certified, processed in FDA-registered facilities
• Triple filtered at 0.1 µm
• Gibco bottle is easier to use in hood, reduces risk of contamination, and helps you perform cell culture more consistently
• Peel-off sticker on label provides handy reference for lot number and expiration date recording in lab notebook

RevitaCell™ Supplement (100X) has been optimized for use with pluripotent stem cells (PSCs), either as a post-thaw recovery solution to improve cell viability or in combination with Essential 8™ Medium to enhance single cell passaging applications. It can also be used for recovery of primary neurons, traditionally a challenging cell to efficiently recover. The significant improvement seen in these applications is due to the presence of a more specific ROCK inhibitor coupled with molecules containing antioxidant and free radical scavenger properties that minimize the impact of stress on the cells.

• • Enabling—provides maximum cell viability when performing single cell passaging

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• • Rapid cell recovery—high post-thaw cell viability and growth of pluripotent stem cells (passaged as clumps or single cells) and neurons

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• • Consistent—formulated using animal origin-free and chemically defined materials for quality and confidence
    
    Single cell passaging
    Use of the RevitaCellTM Supplement for 18-24 hours post-single cell passaging provides vast improvement in cell survival. Cells are shown to reach passaging confluency within 3-4 days post-seeding.
    
    Higher specificity
    Kinase profiling of pro-survival small molecules in RevitaCell™ Supplement shows higher specificity in comparison to traditional ROCK inhibitors (i.e., Y-27632 and Thiazovivin).
    
    Quicker post-thaw recovery
    PSCs recovered at 40,000 viable cells/cm² using media supplemented with RevitaCell™ Supplement for the first 24 hours post-thaw recovery followed by feeding with unsupplemented Essential 8™ Medium will typically reach 80% confluency in <5 days, significantly reducing the average post-thaw recovery time of most cryopreservation solutions. Neurite outgrowth of neurons is also significantly improved using RevitaCell™ Supplement.

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