100 mL

Used for cell dissociation, routine cell culture passaging, and primary tissue dissociation due to its digestive strength.

0.25% Trypsin and 1mM EDTA in modified HBSS w/o Ca++ and Mg++, 100 mL

1-Hexanol

1,1,1,3,3,3-Hexafluoro-Isopropanol

Sodium pyruvate is used by cells as an easily accessible carbohydrate source. The 100 mM solution should be diluted 1:100 for most cell culture.

100X Non-essential Amino Acids for Minimum Essential Medium Eagle Contains 19 amino acids. The essential amino acids and the non-essential amino acids; L-ala; L-asn; L-asp; L-glu; L-gly; L-pro and L-ser.

Trypsin is a porcine pancreas-derived enzyme that is commonly used for the dissociation and disaggregation of anchorage-dependent mammalian cells and tissues.

Ready to use solution. Cell culture grade

Also known as DME, Dulbecco’s Modified Eagle’s Medium is the most widely used modification of Eagle’s Basal Medium (BME).

McCoy’s 5A medium, as modified by Park & Terasaki.

Phosphate Buffered Salts, Dulbecco’s Formula (DPBS) is a balanced salt solution (BSS) used for the handling and culturing of mammalian cells. DPBS is used to irrigate, wash, and dilute mammalian cells. Phosphate buffering maintains the pH in the physiological range. Calcium and magnesium facilitate cell binding and clumping. DPBS without these ions can be used to wash and rinse suspended cells.

RPMI 1640 medium is a general purpose medium based on the RPMI 1630 line of media which utilized a bicarbonate buffering system and alterations in the amounts of amino acids and vitamins. A Dutch Modification of RPMI 1640 is also available through MP. The Dutch Modification contains 6400 mg/L sodium chloride instead of 6000 mg/L; contains 1000 mg/L sodium bicarbonate instead of 2000 mg/L; and contains 20 mM HEPES.

Cytoprotective, protein free, ready-to-use bio-solution without dimethyl sulfoxide (DMSO) for the short-term storage of cells or tissue.

2-Mercaptoethanol

2-Mercaptoethanol

2-Phenoxyethanol

2,2,4,4,6,8,8-Heptamethylnonane

2,2′-Azino-Bis-(3-Ethyl-Benzthiazoline-6-Sulfonic Acid) Solution

3-Aminopropyl-Triethoxysilane

TMB is a chromogenic substrate for Horse Radish Peroxidase (HRP). MP Biolomedicals TMB Solution is a convenient, non toxic and ready to use stable chromogen solution suitable for peroxidase and pseudo-peroxidase reactions in ELISA, it provides consistent performance and sensitivity to deliver reproducible results

This media is selective for growth of cells with a functioning HGPRT mechanism. The main application is for the selective growth of hybridoma cells for monoclonal antibody production. (50X, 13.61 mg/L hypoxanthine, 3.876 mg/L thymidine) 

Sterile 80% Glycerol solution. Less viscous and more convenient to pipette than 100% glycerol. Suitable for cell culture. For cryopreservation of cells

Novel trypsin replacement that exhibits both protease and collagenolytic activities for cell detachment. Effective in detaching primary fibroblasts, endothelial cells, neurons, tumor cell lines, and insect cells. Maintains most cell surface antigens

Acetone

Ammonium Acetate, 7.5 M Solution

Ammonium Sulfide Solution

100 x vitamin solution without glutamine for Basal Medium Eagle.

(5-Bromo-4-chloro-3-indolyl Phosphate/Nitroblue Tetrazolium)Stabilized Chromogen SolutionSpecially prepared for immunoblotting procedures only. Concentration: 0.577 mmol/liter BCIP; 0.122 mmol/liter NBT.

BCIP/NBT Liquid Substrate Plus is a stabilized chromogen solution. It has a greater sensitivity and produces little or no background staining compared with other alternatives. It is ready to use solution.

Stabilized Chromogen Solution
This alkaline phosphatase chromogen solution contains the two components in a stabilized solution which may be used as the final step in color development. The resulting product is a permanent, deep-purple stain. It enhances results in both ELISA and immunohistology assays utilizing peroxidase and alkaline phosphatase.

Benzyl Alcohol