1kit

The gel opener is used to open ExpressPlusᵀᴹ or SurePAGEᵀᴹ gel cassette after gel electrophoresis.

Each kit contains 2x 1L eBlot L1 PVDF Membrane Transfer Buffer (5X), 2x 15mL eBlot L1 PVDF Membrane Equilibration Buffer (10X), 2x 30pK eBlot L1 Transfer Sponge and 2x 15pK eBlot L1 PVDF Membrane. The kit is for 30x transfers with standard program.

eBlot L1 is a highly efficient wet protein transfer system, which uses a patented technology developed by GenScript. eBlot L1 combines the high stability and efficiency of the traditional wet transfer with the rapidness and convenience of the semi-dry transfer system. The device allows the efficient transfer of small, medium as well as large molecular weight proteins within 15 minutes.

Each kit contains 2x 1L eBlot L1 NC Membrane Transfer Buffer (5X), 2x 15mL eBlot L1 NC Membrane Equilibration Buffer (10X) and 2x 30pk eBlot L1 Transfer Sponge. The kit is for 30x transfers with standard program.

eBlot L1 is a highly efficient wet protein transfer system, which uses a patented technology developed by GenScript. eBlot L1 combines the high stability and efficiency of the traditional wet transfer with the rapidness and convenience of the semi-dry transfer system. The device allows the efficient transfer of small, medium as well as large molecular weight proteins within 15 minutes.

Each kit contains 2x 1L eBlot L1 PVDF Membrane Transfer Buffer (5X), 2x 15mL eBlot L1 PVDF Equilibration Buffer (10X ) and 2x 30pK eBlot L1 Transfer Sponge. The kit is for 30x transfers with standard program.

GenScript’s eZwest™ Diluent Kit is designed to be used in combination with the eZwest™ Lite Automated Western Device.
The eZwest™ Diluent Kit speeds up the overall western blotting
process by shortening the incubation time for primary and secondary antibodies.
Moreover, it can be used as a blocking reagent during the incubation, thus
eliminating the need for a separate blocking step. The results obtained using
the eZwest™ Diluent Kit are as sensitive as those obtained through
a conventional western blotting protocol.

The Diluent Kit
should be stored at 2-8℃.

Glutathione Resin (Cat. No. L00206) is an affinity chromatography medium designed for easy, one-step purification of recombinant glutathione S-transferase (GST) fusion proteins and other glutathione binding proteins expressed in E. coli, insect cells and mammalian cells. The recombinant GST fusion proteins can be purified directly from pre-treated cell lysate using Glutathione Resin. It is the excellent choice for high performance purifications. 

GST Fusion Protein Purification Kit (Cat. No. L00207) is the derivate products of Glutathione Resin which includes five disposable columns and five 0.154 g samples of glutathione to facilitate protein purification.

GenScript His Tag ELISA Detection Kit, a 1.5 hour competition ELISA, is developed for rapid and high throughput detection of His-tagged
proteins. There are several potential usages:

1. Quickly identify the presence of His-tagged proteins in samples.
2. Optimize protein expression by monitoring the His-tagged proteins level.
3. High throughput screening of stable cell lines expressing His-tagged proteins.

This kit is based on competitive ELISA method. His Tag Plate in the kit is a 96-well microtiter
plate coated with a His-tagged protein with the molecular weight of 12.7 kDa. The
plate, which comprises of 8 wells x 12 strips, is demountable. When Anti-His Monoclonal
Antibody and His-tagged proteins are added to the well , the coated His-tagged
proteins compete with His Tag Standard (His-tagged protein with the molecular weight of 11.3
kDa) in solution or His-tagged
protein in sample to interact with Anti-His Monoclonal Antibody. The higher the
concentration of the His-tagged protein in solution is, the less the antibody
bound to the plate will be. The Anti-His Monoclonal Antibody used in the kit is
a mouse anti His tag monoclonal antibody (GenScript A00186). Antibody Tracer, which is a horseradish peroxidase (HRP) conjugated Goat anti mouse IgG, is used for enzyme reaction. His-tagged protein, Anti-His Monoclonal Antibody and antibody tracer form a complex. Other unbound molecules can be removed by washing
solution. The antibody tracer reacts with TMB substrate to develop blue
product that turns yellow immediately when the Stop Solution is added, which
can be measured by microplate reader at 450 nM.

In optimized
test condition, each absorbance value is indicated to the individual His-tagged
proteins amount in solution.  His-tagged
protein standards of known concentration and the corresponding absorbance
values are used to construct a standard curve. With the standard curve, His-tagged
protein amount present in the unknown sample can be calculated by transforming
its absorbance value. 

Protein A affinity chromatography is widely used for antibody purification. However, the leakage of Protein A, though covalently conjugated to chromatography supports, posts a big problem for antibody based pharmaceutical industry where false results could be generated in immunological assays from contaminated Protein A.

GenScript Protein A ELISA Kit
is developed for accurate quantification of the leakage of native Protein A, recombinant Protein A variants and MabSelect SuRe ^TM
in the presence or absence of antibodies. The kit offers pharmaceutical users a sensitive assay to measure mini amount of Protein A in therapeutic antibody products. It also facilitates Protein A resin manufacturers in monitoring leaching characteristics of their resins under specific conditions.

Key Features:

• Accurate detection of 20 pg/ml Protein A and 50 pg/ml MabSelect SuRe ^TM
  in antibody preps
• Broad-range recongnition of native Protein A, recombinant Protein A variants and MabSelect SuRe
  ^TM
• Low level deviation – Intra-assay
  <5%, Inter-assay <10%
• High accuracy/recovery within 85-115%
• Ready-to-use ELISA plate with fast & simple samples preparation
• Time-saving protocol – complete assay in less than 2 hours
• Exceptional lot-to-lot consistency ensured by high-quality monoclonal antibodies
• Wide applications for the quantification of Protein A contaminant and leaching

Storage:

The kit is stable for 12 months if stored at 2-8 &#8451.
Do NOT freeze any component of the kit.

Roller for eBlot

GenScript ToxinEraser™ is an endototoxin removal resin of high efficiency. It is based on the affinity matrix of modified polymyxin B (PMB), which allows highly efficient endotoxin removal. The final endotoxin level can be decreased to less than 0.1 EU/ml with repeat use of ToxinEraser™ endotoxin removal resin. Final removal efficiency may vary depending on the sample type/source.

GenScript ToxinSensor™ Gel Clot Endotoxin Assay
Kit is designed as a simple and sensitive in vitro end-product endotoxin test
for human and animal parenteral drugs, biological products, and medical
devices. The LAL test is a qualitative test for Gram-negative bacterial endotoxin.
Lyophilized Amebocyte Lysate as supplied in the kit is to be reconstituted with
LAL Reagent Water and then mixed in equal parts with the solution being tested.
After incubation, and in the presence of endotoxin, gelation occurs; in the
absence of endotoxin, gelation does not occur. Lyophilized Amebocyte Lysate
reagent is made from amebocyte lysate from the horseshoe crab (Tachypleus tridentatus).The protocol
described herein conforms to those described in the FDA guideline. Similar
performance requirements for gel clot assays have been published and are
updated regularly in the United States Pharmacopeia.