20 mL

Amphotericin B is a polyene antifungal antibiotic from Streptomyces sp. It has a high affinity for sterols, primarily ergosterols, of fungal and bacterial cell membranes. It is an antibiotic solution effective against yeast and other fungi. Amphotericin B may be toxic to some insect cell types. Amphotericin B, is an antimycotic.

Cellvation Cryopreservation Media

Collagen Solution

DNAse free water for use in DNA extraction and purification

G418 Sulfate is an aminoglycoside related to Gentamicin and is toxic to bacteria, yeast, higher plants, mammalian cells, protozoans and helminths. G418 Sulfate is an effective selecting agent utilized in killing prokaryotic and eukaryotic cells.

Resistance to Geneticin Selection Antibiotic (G418 Sulfate) is conferred by the E. coli APH(3)–I and APH(3)–II resistance genes.

• • Used as selective antibiotic in concentration range of 100 – 200µg/mL for bacteria, or 200 – 500µg/mL for most mammalian cells

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• • Supplied as 50mg/mL solution in water

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Cell Culture, Cloning, Drosophila S2 Cell Culture, High Five™ Cell Culture, Insect Cell Culture, Mammalian Cell Culture, Selection, Sf9 & Sf21 Cell Culture, Transfection, Transformation

Pimaricin

Double antibiotic solution containing 10,000 units/mL of penicillin and 10,000 µg/mL of streptomycin.

For use as blocking agent in immunohistochemistry and a a component of cell culture media.

Red blood cells collected into Alsever’s solution. Washed with phosphate buffered saline. Preserved with borate buffer, sodium acetate and 0.1% thimerosal

TCH® is a fortified, multipurpose serum replacement for long-term culturing of primarily human cells and the production of cell secreted proteins. TCH is packaged as a 50X concentrate.

TCM™ is a fortified, multipurpose serum replacements for the long-term culturing of anchorage dependent and suspension cells from a variety of species. TCM™ is packaged as a 50X concentrate.

Description

Thermo Scientific™ SuperSignal West Femto Substrate is an ultra-sensitive enhanced chemiluminescent substrate (ECL substrate) for detection of peroxidase activity from HRP-conjugated antibodies and probes in Western blotting.

nSuperSignal West Femto Substrate Maximum Sensitivity Substrate is an ultra-sensitive chemiluminescent substrate system for Western blotting with horseradish peroxidase (HRP) enzyme. The substrate kit enables detection of low femtogram (that’s mid-zeptomole!) amounts of protein deposited on nitrocellulose or PVDF membrane and probed with appropriate primary and secondary antibodies. When combined with optimized antibody concentrations and blocking buffers, SuperSignal West Femto Substrate enables detection of target proteins in amounts that are small to be seen with typical ECL substrates.nnHighlights:n

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  • ECL substrate – an enhanced chemiluminescent substrate for detection of horseradish peroxidase (HRP) activity from antibodies and other Western blot probes

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• • Sensitive – detect low-femtogram (mid-zeptomole) amounts of protein in bands on nitrocellulose or PVDF membranes

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• • Quantitative – produces quantitative signal that is measurable over two orders of magnitude

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• • Saves on antibody – optimized for very dilute antibody concentrations; dilute primary antibodies 5000- to 100,000-fold and secondary antibodies 100,000 to 500,000-fold (from 1mg/mL stocks)

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• • Intense signal – easy to capture an image by exposure to autoradiography film or via a CCD camera or similar imaging system

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• • Excellent signal duration – 8 hours of usable light-output when conditions are optimized

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• • Stable reagent – kit components are stable for 1 year at 4°C or 6 months at room temperature

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