25ml Resin (Total 50 ml)

GenScript Anti-DYKDDDDK Affinity Resin Easy (Cat. No. L00907) features high binding capacity, specificity, sensitivity, stability, and reusability, making it particularly useful for the affinity purification and immunoprecipitation of DYKDDDDK-tagged fusion proteins expressed in common protein expression systems such as bacteria, yeast, and mammalian cells.

GenScript Anti‐DYKDDDDK G1 Affinity Resin (Cat. No. L00432) is designed for the purification of DYKDDDDK‐tagged protein from commonly used protein expression systems including bacteria, yeast and mammalian cells.

This product is designed for purification of proteins, antibodies and compounds containing (GnS)m linker (n≥2, m≥2) from commonly used protein expression systems such as bacteria, yeast and mammalian cells.

GenScript
High Affinity Ni-Charged Resin is an 4% cross-linked agarose medium covalently
coupled to a chelating agent that binds Ni2+ by four coordination
sites for high-affinity purification of polyhistidine-tagged recombinant
proteins. High Affinity Ni-Charged Resin has
low Ni²⁺ leakage, high protein-binding capacity and stability, and
is compatible with a wide range of additives used in protein purification. This
makes High Affinity Ni-Charged Resin the excellent choice for high performance
purification of polyhistidine-tagged proteins. 

Camelid
VHH Antibody (also called single-domain antibody, sdAb or Nanobody by Ablynx)
is a peptide chain about 110 amino acids in length, containing one variable domain
of a heavy-chain antibody (a VHH fragment). Like a whole antibody, the camelid
VHH antibody is able to bind selectively to a specific antigen. This single-domain
antibody has a broad range of applications in biotechnical and therapeutic research
fields due to its small size, simple production and high affinity.
GenScript’s MonoRab™ Anti-Camelid VHH
Affinity Resin (Cat. No. L00905) is designed for purification of Camelid VHH
single domain antibody from commonly used protein expression systems such as bacteria,
yeast and mammalian cells. The VHH single domain antibody in cell lysate can specifically
bind to the Anti-Camelid VHH monoclonal antibody coupled to the resin. Nonspecific
binding reactions are eliminated by stringent washing steps, and the purified Camelid
VHH single domain antibody is eluted with a high recovery rate. Table
1 lists the main characteristics of GenScript’s MonoRab™ Anti-Camelid VHH
Affinity Resin.

 Table 1. Characteristics of Anti-Camelid VHH Affinity
Resin

GenScript’s MonoRabᵀᴹ Anti-DYKDDDDK Affinity Resin is designed for optimal purification of DYKDDDDK-tagged proteins. DYKDDDDK tag is an octapeptide tag that can be added to a protein using recombinant DNA technology. It can be fused to the N-terminus or C-terminus of a target protein to facilitate detection and purification. Based on our new rabbit monoclonal antibody technology (MonoRabᵀᴹ), this resin offers high binding capacity, high purity, and resistance to high concentrations of salt or acid during protein purification. The binding capacity of MonoRabᵀᴹ Anti-DYKDDDDK Affinity Resin is 1.5 mg DYKDDDDK-tagged protein per mL of settled resin. The recombinant proteins expressed in bacterial or mammalian cells cultures specifically bind to the anti-DYKDDDDK monoclonal antibodies coupled to the resin, resulting in highly pure eluates. The resin can withstand stringent washing steps, especially with buffers containing high concentration of salt. The resin is resistant to up to 2 M NaCl, and to buffers with pH as low as 2.5.

GenScript Ni Resin FF is a 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni²⁺ by three coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. Ni Resin FF has low Ni²⁺ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. Ni Resin FF is an excellent choice for high performance purification of polyhistidine-tagged proteins.

GenScript Protein G Resin FF is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. The recombinant protein G ligand is coupled to 4% highly cross-linked agarose. The static binding capacity of Protein G Resin FF is greater than 20 mg human IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc. Table 1 lists the characteristics of Protein G Resin FF.
Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native protein G has 3 IgG binding domains and also sites for albumin and cell-surface binding. The latter have been eliminated from recombinant protein G to reduce nonspecific binding. Although protein G has very similar tertiary structures to protein A, their amino acid compositions differ significantly, resulting in different binding characteristics. Protein G can be used for purification of mammalian monoclonal and polyclonal IgGs that do not bind well to protein A. Protein G has greater affinity than protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike protein A, protein G does not bind to human IgM, IgD and IgA.

Storage

Characteristics