50 mL

From bovine blood collected at a USDA licensed facility. From cattle free from infectious and contagious diseases

Globulin free albumin. Suitable for elctrophoresis and western blotting

Albumin bovine path 5 is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7 it contains 17 intrachain disulfide bridges and 1 sulfhydryl group.

Catalase

Cell Cryopreservation Medium with 10% DMSO is a balanced, protein free, ready-to-use cryopreservation solution with 10% USP DMSO (Cryopres™). It does not require any dilution or further processing and can be used for cells cultured in serum free medium or with serum containing medium.

CHAPS Lysis Buffer

CHAPS Lysis Buffer (2X)

Cineole

Cryopres Dimethyl Sulfoxide is commonly used in cell cryopreservation, along with glycerol, to prevent damage to the cell membrane during freezing.

Diethyl Pyrocarbonate

Dimethyl Sulfoxide

E. coli Sample Buffer

FBS, Heat-Inactivated, US Origin

FBS, US Origin

FBS, USDA approved

Freund’s Complete Adjuvant, Mycobacterium butyricum, consists of heat-killed Mycobacterium butyricum in non-metabolizable oils (paraffin oil) and surfactant (Arlacel A). Components are thoroughly mixed in controlled proportions and autoclaved prior to being vialed.

Features of Freund’s Complete Adjuvant (FCA):

• Also called Complete Freund’s Adjuvant (CFA).
• FCA can be utilized to produce water-in-oil emulsions of antigens.
• FCA can significantly enhance the immune response to immunogens by inducing a potent and long-lasting immune response.
• FCA contains heat-killed Mycobacterium butyricum, which help to stimulate the immune system.
• FCA is often used for initial immunization.

This product is made by a modification of Freund’s technique, in which 25 mg of Mycobacterium is suspended in a mixture of 7.5 mL Arlacel A and 42.5 mL paraffin oil. An adjuvant is a material that when administered enhances the antibody response of animals injected with antigen.

Freund’s incomplete adjuvant is used for the boosts. Prepared from non-metabolizable oils (paraffin oil and mannide monooleate)

G418 Sulfate is an aminoglycoside related to Gentamicin and is toxic to bacteria, yeast, higher plants, mammalian cells, protozoans and helminths. G418 Sulfate is an effective selecting agent utilized in killing prokaryotic and eukaryotic cells.

Wash solution for use with GENECLEAN SPIN Glassmilk.

For many years, researchers have used a common recipe for making freezing medium: High-glucose Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% serum and 10% DMSO. Recovery Cell Culture Freezing Medium is an optimized version of this classic formulation, using Gibco Bovine Serum and Fetal Bovine Serum to provide improved cell recovery and viability after thawing.

• • Suitable for a wide variety of mammalian cells

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• • Can be used to freeze most mammalian cells cultured in DMEM, DMEM/F-12, MEM, RPMI 1640, and Ham’s F-12

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• • Quality tested for pH, osmolality, sterility, and endotoxin

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• • Each lot is performance tested using CHO-K1 cells

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Cell Counting, Viability & Cryopreservation, Cell Culture, Mammalian Cell Culture, Gene Expression Analysis & Genotyping, In Vitro Transcription, PCR & Real-Time PCR

Normal goat serum is commonly used for the blocking of non-specific antibody binding in tissue and cell staining, and in other applications of antibodies. It can successfully reduce nonspecific binding of proteins and antibodies to reaction surfaces and non-specific binding sites. It provides an alternative to bovine serum albumin (BSA) and non-fat dry milk.

10% Solution
Prepared from Fraction V human albumin, this solution is supplied at a pH of 7.1 ±0.3 and contains 0.05% sodium azide as a preservative.
See the Immunobiologicals Section for additional details.

Human Platelet Lysate (Heparin Free)

IEF Sample Buffer (2X) pH 3-10, with lysine and arginine

IEF Sample Buffer (4X) pH 3-7, with lysine

Igepal® Ca-630

Kanamycin is an aminoglycoside antibiotic effective against mycoplasma, gram-positive and gram-negative bacteria. It is used in biotechnology applications to inhibit protein synthesis.

Sample loading buffer for protein gel electrophoresis.