500 mL

General purpose medium based on the RPMI 1640 line of media. A Dutch Modification of RPMI 1640 is also available through MP

Roswell Park Memorial Institute (RPMI) 1640 Medium is suitable for a variety of mammalian cells, including HeLa, Jurkat, MCF-7, PC12, PBMC, astrocytes, and carcinomas.

• • RPMI is modified as follows: with L-glutamine, phenol red; without HEPES

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• • Unique from other media because it contains reducing agent glutathione and high concentrations of vitamins

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• • Contains biotin, vitamin B₁₂, and PABA, which are not found in Eagle’s Minimal Essential Medium or Dulbecco’s Modified Eagle Medium

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• • Vitamins inositol and choline are present in high concentrations

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• • Contains no proteins, lipids, or growth factors

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• • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS)

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• • Uses sodium bicarbonate buffer system (2.0g/L), and therefore requires a 5-10% CO₂ environment to maintain physiological pH

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• • Sustainable packaging

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Cell Culture, Mammalian Cell Culture

RPMI 1640 without L-Glutamine

Safflower Oil

Salicylaldehyde, 99.5%

SDS Easy Solution (12%)

Separating or Resolving Buffer (4X)

Serum Free Media-293

Serum Free Media-CHO

Serum Free Media-Doma

Serum Free Media-HeLa

Serum Free Media-hESC

Serum Free Media-hHSC

Serum Free Media-hMSC

Serum Free Media-NSO

This serum is processed from whole blood collected from a controlled herd of young horses. Each animal is routinely screened for Equine Infectious Anemia (EIA). Donor Horse Serum is suitable for use in diagnostic assays, as a supplement in mycoplasma growth and assay media and for culturing hematopoietic stem cells and neuronal cells.

This serum is obtained from pooled, normal clotted human blood.

This serum is derived from whole blood of healthy young lambs.

This serum is produced from blood collected from newly born calves between 1-14 days of age.

SM buffer (sterile solution)

Sodium Dodecyl Sulfate Solution

Component of the FastDNA SPIN Kit for Soil and FastDNA SPIN Kit for Feces.

Squalene

20xSSC solution for use in Southern and northern blotting.

SSC Buffer (20X), RNase-free

SSPE (20X), RNase-free

Stacking Buffer (4X)

Used for the growth and maintenance of living cells. To maintain yeast cells prior to induction of expression of genes that are regulated by the GAL1 promoter by galactose.

Sucrose, 80% in MNE Buffer (MES + NaCl + EDTA)

SupraGLN™, (pronounced soo-pra gloo-tuh-meen), is a stabilized form of L-glutamine, which is a key component of mammalian cell culture media. SupraGLN serves as a major energy source for cell proliferation and is able to support overall cell health by promoting a more efficient metabolism.

Thermo Scientific™ Pierce BCA Protein Assay Kit remains one of the most popular protein quantitation methods worldwide.

Prevent chemical accidents in the lab with these FEP Wash Bottles. Bottles labeled following the Globally Harmonized System (GHS).

Prevent chemical accidents in the lab with Nalgene™ Right-to-Understand FEP Wash Bottles. Bottles are permanently etched with chemical identification and safe handling information following the Globally Harmonized System (GHS) specifications as applicable to secondary laboratory containers. Wash bottles are ready to use when you transfer harsh laboratory chemicals from manufacturers’ containers. Six bottles available. For use with methyl ethyl ketone; n-hexane; toluene; xylene; methylene chloride; acetone.

Our variety of Pierce Concentrated Buffer stock solutions are ready to use without having to weigh and dissolve dry ingredients or adjust the pH with concentrated acid or base. Simply dilute the liquid stock solution with pure water and proceed with your experiment.

nBenefits of using Pierce Concentrated Buffer stock solutions include:
• Convenient—no weighing, no pH adjustment, no need to stock individual components
• Improved accuracy—ready-to-use formula, easily diluted without weighing chemical components
• Consistent quality—our quality control ensures that every prep will yield the same, consistent buffer

20X PBS Tween-20 can be used to prepare PBS-Tween (PBS-T) wash buffers for ELISA, western, and other immunoassays, as well as blocking buffer for plate-based assays. When diluted to 1X with water, 20X PBS Tween-20 makes 10 mM sodium phosphate, 0.15 M NaCl, 0.05% Tween™ 20 buffer at pH 7.5.

20X TBS Tween-20 (Tris-buffered saline with Tween™ 20 detergent) can be used to prepare TBS-Tween (TBS-T) wash buffers or blocking buffer diluent for applications such as ELISA, western blotting, and other immunoassays. When diluted 20-fold in water, a TBS Tween-20 buffer containing 25 mM Tris, 0.15 M NaCl, 0.05% Tween-20 at pH 7.5 is ready for use.

20X Tris-Buffered Saline (TBS) is a stock solution for preparing Tris-NaCl buffer for use as a wash buffer and antibody diluent for ELISA, western blotting, and other immunoassays. 20X Tris Buffered Saline (TBS) makes 25 mM Tris, 0.15 M NaCl with pH 7.2 to 7.5 when diluted to 1X with water.

20X Phosphate-Buffered Saline (PBS) is ideal for preparing PBS buffers for use in crosslinking, biotinylation, and fluorescent labeling reactions requiring an amine-free buffer. When diluted 20-fold in water, the solution yields 10 mM sodium phosphate, 0.15 M NaCl at pH 7.5.

10X Tris-Glycine-SDS Buffer makes 0.025 M Tris, 0.192M glycine, 0.1% sodium dodecyl sulfate (SDS) at pH 8.5 when diluted to 1X with water.

20X Borate Buffer is ideal for preparing sodium borate buffer solutions for use in protein modification procedures requiring amine-free buffer at an alkaline pH. 20X Borate Buffer makes 50 mM borate at pH 8.5 when diluted to 1X with water.

20X Modified Dulbecco’s PBS is used for preparing physiological Na- and K-phosphate buffered saline (D-PBS) used for wash buffers and antibody diluents in immunoassays like ELISA and western blot. 20X Modified Dulbecco’s PBS makes 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14 M NaCl, 10 mM KCl, pH 7.4 when diluted to 1X with water.

10X Tris-Glycine Buffer is a space-saving stock solution that is ideal for quickly preparing standard Tris-glycine (pH 8.5) transfer buffer used for western blotting using Tris-glycine gel electrophoresis. When diluted 10-fold in water or 20% methanol, the solution yields 0.025 M Tris, 0.192 M glycine at pH 8.5.

10X TBE Buffer stock solution is ideal for casting and preparing Tris-borate-EDTA (TBE) running buffer used for agarose and polyacrylamide gel electrophoresis of nucleic acids. When diluted to 1X, the TBE Buffer contains 0.089 M Tris, 0.089 M borate, 2 mM EDTA with pH 8.2 to 8.4.

20X Modified Dulbecco’s PBS Tween-20 will make physiological sodium- and potassium-phosphate buffered saline (D-PBS) to be used as a wash buffer for ELISA, western, and other immunoassays or as diluent or blocking buffer for plate-based assays. 20X Modified Dulbecco’s PBS Tween-20 (D-PBS-T) makes 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14 M NaCl, 10 mM KCl, 0.05% Tween™ 20 at pH 7.4 when diluted to 1X with water.

20X TAE Buffer is a space-saving stock solution that is ideal for casting and preparing Tris-acetate EDTA (TAE) running buffer for agarose gel electrophoresis of nucleic acids. When diluted to 1X with water, a 0.04 M Tris, 0.04 M acetate, 1 mM EDTA buffer at pH 8.2 to 8.4 is ready for use.

Our variety of Pierce Concentrated Buffer stock solutions are ready to use without having to weigh and dissolve dry ingredients or adjust the pH with concentrated acid or base. Simply dilute the liquid stock solution with pure water and proceed with your experiment.

nBenefits of using Pierce Concentrated Buffer stock solutions include:
• Convenient—no weighing, no pH adjustment, no need to stock individual components
• Improved accuracy—ready-to-use formula, easily diluted without weighing chemical components
• Consistent quality—our quality control ensures that every prep will yield the same, consistent buffer

20X PBS Tween-20 can be used to prepare PBS-Tween (PBS-T) wash buffers for ELISA, western, and other immunoassays, as well as blocking buffer for plate-based assays. When diluted to 1X with water, 20X PBS Tween-20 makes 10 mM sodium phosphate, 0.15 M NaCl, 0.05% Tween™ 20 buffer at pH 7.5.

20X TBS Tween-20 (Tris-buffered saline with Tween™ 20 detergent) can be used to prepare TBS-Tween (TBS-T) wash buffers or blocking buffer diluent for applications such as ELISA, western blotting, and other immunoassays. When diluted 20-fold in water, a TBS Tween-20 buffer containing 25 mM Tris, 0.15 M NaCl, 0.05% Tween-20 at pH 7.5 is ready for use.

20X Tris-Buffered Saline (TBS) is a stock solution for preparing Tris-NaCl buffer for use as a wash buffer and antibody diluent for ELISA, western blotting, and other immunoassays. 20X Tris Buffered Saline (TBS) makes 25 mM Tris, 0.15 M NaCl with pH 7.2 to 7.5 when diluted to 1X with water.

20X Phosphate-Buffered Saline (PBS) is ideal for preparing PBS buffers for use in crosslinking, biotinylation, and fluorescent labeling reactions requiring an amine-free buffer. When diluted 20-fold in water, the solution yields 10 mM sodium phosphate, 0.15 M NaCl at pH 7.5.

10X Tris-Glycine-SDS Buffer makes 0.025 M Tris, 0.192M glycine, 0.1% sodium dodecyl sulfate (SDS) at pH 8.5 when diluted to 1X with water.

20X Borate Buffer is ideal for preparing sodium borate buffer solutions for use in protein modification procedures requiring amine-free buffer at an alkaline pH. 20X Borate Buffer makes 50 mM borate at pH 8.5 when diluted to 1X with water.

20X Modified Dulbecco’s PBS is used for preparing physiological Na- and K-phosphate buffered saline (D-PBS) used for wash buffers and antibody diluents in immunoassays like ELISA and western blot. 20X Modified Dulbecco’s PBS makes 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14 M NaCl, 10 mM KCl, pH 7.4 when diluted to 1X with water.

10X Tris-Glycine Buffer is a space-saving stock solution that is ideal for quickly preparing standard Tris-glycine (pH 8.5) transfer buffer used for western blotting using Tris-glycine gel electrophoresis. When diluted 10-fold in water or 20% methanol, the solution yields 0.025 M Tris, 0.192 M glycine at pH 8.5.

10X TBE Buffer stock solution is ideal for casting and preparing Tris-borate-EDTA (TBE) running buffer used for agarose and polyacrylamide gel electrophoresis of nucleic acids. When diluted to 1X, the TBE Buffer contains 0.089 M Tris, 0.089 M borate, 2 mM EDTA with pH 8.2 to 8.4.

20X Modified Dulbecco’s PBS Tween-20 will make physiological sodium- and potassium-phosphate buffered saline (D-PBS) to be used as a wash buffer for ELISA, western, and other immunoassays or as diluent or blocking buffer for plate-based assays. 20X Modified Dulbecco’s PBS Tween-20 (D-PBS-T) makes 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14 M NaCl, 10 mM KCl, 0.05% Tween™ 20 at pH 7.4 when diluted to 1X with water.

20X TAE Buffer is a space-saving stock solution that is ideal for casting and preparing Tris-acetate EDTA (TAE) running buffer for agarose gel electrophoresis of nucleic acids. When diluted to 1X with water, a 0.04 M Tris, 0.04 M acetate, 1 mM EDTA buffer at pH 8.2 to 8.4 is ready for use.

Our variety of Pierce Concentrated Buffer stock solutions are ready to use without having to weigh and dissolve dry ingredients or adjust the pH with concentrated acid or base. Simply dilute the liquid stock solution with pure water and proceed with your experiment.

nBenefits of using Pierce Concentrated Buffer stock solutions include:
• Convenient—no weighing, no pH adjustment, no need to stock individual components
• Improved accuracy—ready-to-use formula, easily diluted without weighing chemical components
• Consistent quality—our quality control ensures that every prep will yield the same, consistent buffer

20X PBS Tween-20 can be used to prepare PBS-Tween (PBS-T) wash buffers for ELISA, western, and other immunoassays, as well as blocking buffer for plate-based assays. When diluted to 1X with water, 20X PBS Tween-20 makes 10 mM sodium phosphate, 0.15 M NaCl, 0.05% Tween™ 20 buffer at pH 7.5.

20X TBS Tween-20 (Tris-buffered saline with Tween™ 20 detergent) can be used to prepare TBS-Tween (TBS-T) wash buffers or blocking buffer diluent for applications such as ELISA, western blotting, and other immunoassays. When diluted 20-fold in water, a TBS Tween-20 buffer containing 25 mM Tris, 0.15 M NaCl, 0.05% Tween-20 at pH 7.5 is ready for use.

20X Tris-Buffered Saline (TBS) is a stock solution for preparing Tris-NaCl buffer for use as a wash buffer and antibody diluent for ELISA, western blotting, and other immunoassays. 20X Tris Buffered Saline (TBS) makes 25 mM Tris, 0.15 M NaCl with pH 7.2 to 7.5 when diluted to 1X with water.

20X Phosphate-Buffered Saline (PBS) is ideal for preparing PBS buffers for use in crosslinking, biotinylation, and fluorescent labeling reactions requiring an amine-free buffer. When diluted 20-fold in water, the solution yields 10 mM sodium phosphate, 0.15 M NaCl at pH 7.5.

10X Tris-Glycine-SDS Buffer makes 0.025 M Tris, 0.192M glycine, 0.1% sodium dodecyl sulfate (SDS) at pH 8.5 when diluted to 1X with water.

20X Borate Buffer is ideal for preparing sodium borate buffer solutions for use in protein modification procedures requiring amine-free buffer at an alkaline pH. 20X Borate Buffer makes 50 mM borate at pH 8.5 when diluted to 1X with water.

20X Modified Dulbecco’s PBS is used for preparing physiological Na- and K-phosphate buffered saline (D-PBS) used for wash buffers and antibody diluents in immunoassays like ELISA and western blot. 20X Modified Dulbecco’s PBS makes 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14 M NaCl, 10 mM KCl, pH 7.4 when diluted to 1X with water.

10X Tris-Glycine Buffer is a space-saving stock solution that is ideal for quickly preparing standard Tris-glycine (pH 8.5) transfer buffer used for western blotting using Tris-glycine gel electrophoresis. When diluted 10-fold in water or 20% methanol, the solution yields 0.025 M Tris, 0.192 M glycine at pH 8.5.

10X TBE Buffer stock solution is ideal for casting and preparing Tris-borate-EDTA (TBE) running buffer used for agarose and polyacrylamide gel electrophoresis of nucleic acids. When diluted to 1X, the TBE Buffer contains 0.089 M Tris, 0.089 M borate, 2 mM EDTA with pH 8.2 to 8.4.

20X Modified Dulbecco’s PBS Tween-20 will make physiological sodium- and potassium-phosphate buffered saline (D-PBS) to be used as a wash buffer for ELISA, western, and other immunoassays or as diluent or blocking buffer for plate-based assays. 20X Modified Dulbecco’s PBS Tween-20 (D-PBS-T) makes 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14 M NaCl, 10 mM KCl, 0.05% Tween™ 20 at pH 7.4 when diluted to 1X with water.

20X TAE Buffer is a space-saving stock solution that is ideal for casting and preparing Tris-acetate EDTA (TAE) running buffer for agarose gel electrophoresis of nucleic acids. When diluted to 1X with water, a 0.04 M Tris, 0.04 M acetate, 1 mM EDTA buffer at pH 8.2 to 8.4 is ready for use.

Our variety of Pierce Concentrated Buffer stock solutions are ready to use without having to weigh and dissolve dry ingredients or adjust the pH with concentrated acid or base. Simply dilute the liquid stock solution with pure water and proceed with your experiment.

Benefits of using Pierce Concentrated Buffer stock solutions include:
• Convenient—no weighing, no pH adjustment, no need to stock individual components
• Improved accuracy—ready-to-use formula, easily diluted without weighing chemical components
• Consistent quality—our quality control ensures that every prep will yield the same, consistent buffer

20X PBS Tween-20 can be used to prepare PBS-Tween (PBS-T) wash buffers for ELISA, western, and other immunoassays, as well as blocking buffer for plate-based assays. When diluted to 1X with water, 20X PBS Tween-20 makes 10 mM sodium phosphate, 0.15 M NaCl, 0.05% Tween™ 20 buffer at pH 7.5.

20X TBS Tween-20 (Tris-buffered saline with Tween™ 20 detergent) can be used to prepare TBS-Tween (TBS-T) wash buffers or blocking buffer diluent for applications such as ELISA, western blotting, and other immunoassays. When diluted 20-fold in water, a TBS Tween-20 buffer containing 25 mM Tris, 0.15 M NaCl, 0.05% Tween-20 at pH 7.5 is ready for use.

20X Tris-Buffered Saline (TBS) is a stock solution for preparing Tris-NaCl buffer for use as a wash buffer and antibody diluent for ELISA, western blotting, and other immunoassays. 20X Tris Buffered Saline (TBS) makes 25 mM Tris, 0.15 M NaCl with pH 7.2 to 7.5 when diluted to 1X with water.

20X Phosphate-Buffered Saline (PBS) is ideal for preparing PBS buffers for use in crosslinking, biotinylation, and fluorescent labeling reactions requiring an amine-free buffer. When diluted 20-fold in water, the solution yields 10 mM sodium phosphate, 0.15 M NaCl at pH 7.5.

10X Tris-Glycine-SDS Buffer makes 0.025 M Tris, 0.192M glycine, 0.1% sodium dodecyl sulfate (SDS) at pH 8.5 when diluted to 1X with water.

20X Borate Buffer is ideal for preparing sodium borate buffer solutions for use in protein modification procedures requiring amine-free buffer at an alkaline pH. 20X Borate Buffer makes 50 mM borate at pH 8.8 when diluted to 1X with water.

20X Modified Dulbecco’s PBS is used for preparing physiological Na- and K-phosphate buffered saline (D-PBS) used for wash buffers and antibody diluents in immunoassays like ELISA and western blot. 20X Modified Dulbecco’s PBS makes 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14 M NaCl, 10 mM KCl, pH 7.4 when diluted to 1X with water.

10X Tris-Glycine Buffer is a space-saving stock solution that is ideal for quickly preparing standard Tris-glycine (pH 8.5) transfer buffer used for western blotting using Tris-glycine gel electrophoresis. When diluted 10-fold in water or 20% methanol, the solution yields 0.025 M Tris, 0.192 M glycine at pH 8.5.

10X TBE Buffer stock solution is ideal for casting and preparing Tris-borate-EDTA (TBE) running buffer used for agarose and polyacrylamide gel electrophoresis of nucleic acids. When diluted to 1X, the TBE Buffer contains 0.089 M Tris, 0.089 M borate, 2 mM EDTA with pH 8.2 to 8.4.

20X Modified Dulbecco’s PBS Tween-20 will make physiological sodium- and potassium-phosphate buffered saline (D-PBS) to be used as a wash buffer for ELISA, western, and other immunoassays or as diluent or blocking buffer for plate-based assays. 20X Modified Dulbecco’s PBS Tween-20 (D-PBS-T) makes 8 mM sodium phosphate, 2 mM potassium phosphate, 0.14 M NaCl, 10 mM KCl, 0.05% Tween™ 20 at pH 7.4 when diluted to 1X with water.

20X TAE Buffer is a space-saving stock solution that is ideal for casting and preparing Tris-acetate EDTA (TAE) running buffer for agarose gel electrophoresis of nucleic acids. When diluted to 1X with water, a 0.04 M Tris, 0.04 M acetate, 1 mM EDTA buffer at pH 8.2 to 8.4 is ready for use.

Thermo Scientific™ Pierce Western Blot Signal Enhancer is a two-reagent system for conditioning protein blots after transfer to greatly enhance the effectiveness of primary antibodies and intensify the final detection signal in Western blot experiments.

The Pierce Western Blot Signal Enhancer membrane treatment procedure is very simple, takes only 15 minutes and can be added to nearly any existing Western blotting protocol. The result is an increase in the intensity of target protein bands on the Western blot or detection of target proteins at levels that were previously not possible. The product is effective for signal intensification with both chemiluminescent and chromogenic substrates, especially with nitrocellulose membranes.

Highlights:

• Increases protein detection – most protein targets show a three- to 10-fold increase in signal intensity, enabling much less protein to be detected with the same substrate and method
• Improves antibody binding – the membrane-treatment reagent exposes and conditions target proteins so that specific antibodies can bind more effectively
• Works for nearly any protein – signal enhancement has been demonstrated with targets such as IL-6, p53, NFkB, BRCA1 and EGF
• Effective with any substrate – enhances both chemiluminescent (ECL) and colorimetric detection for Western blots
• Compatible with any membrane – enhances signal on nitrocellulose and PVDF membrane, regardless of pore size (enhancement is less pronounced with PVDF)
• Fast, 15-minute protocol – optimized for a combination of simplicity, speed and signal enhancement for most proteins
• Ready-to-use – no formulating or diluting necessary, and the reagents are stable for storage at room temperature

Includes:

Enhancer Reagent 1, 250mL; Enhancer Reagent 2, 250mL

TN Buffer Solution

Tri-N-octylamine

Triacetin

Tributyrin

Trichloroacetic acid, 6.1 N solution

Triethanolamine

Trifluoroacetic Acid

Tris HCl buffer 1M solution pH 8

TRIS-Acetate-SDS Running Buffer (10X), pH 8.3

TRIS-CAPS Buffer (10X)

TRIS-HEPES-Native Running Buffer (10X), pH 8.0