5ml x 5
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High Affinity Ni-Charged Resin FF Prepacked Column
$398.48 Add to cart View Product DetailsGenScript High Affinity Ni-Charged Resin FF is an 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin FF has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin FF Prepacked Column the excellent choice for high performance purification of polyhistidine-tagged proteins.
Cat. No. Product Name Volume supplied Resin content L00683-12 High Affinity Ni-charged Resin FF prepacked column (1mlx2) 1mlx2 prepacked column L00683-51 High Affinity Ni-charged Resin FF prepacked column (5mlx1) 5mlx1 L00683-55 High Affinity Ni-charged Resin FF prepacked column (5mlx5) 5mlx5 Storage
Store in 20% ethanol at 2°C to 8°C. Do not freeze.Characteristics
Binding capacity >40 mg of histidine-tagged protein /ml settled resin Matrix spherical 6% highly cross-linked agarose Average particle size 90 μm (45-165 μm) Storage solution 20% ethanol Storage and Stability 18 months when stored unopened at 2-8 °C Column compatibility
The 5 mL and 1 mL columns are compatible with ÄKTA™ chromatography systems. Additional adaptors may be needed for other systems. The optimal flow rate is 1 mL/min for the 1 mL column, and 5 mL/min for the 5 mL column. For preserving the column integrity, please do not exceed a flow rate of 4 mL/min for the 1 mL column and 15 mL/min for the 5 mL column (The mobile phase of the test is aqueous buffers at room temperature and the alarm pressure is 0.3 MPa).Reagents Compatible
Denaturants Detergents Reducing agents Salts Others 6 M Gu·HCl 2% Triton X-100 20 mM β-ME 4 M MgCl2 50% glycerol 8 M Urea 2% Tween 20 1 mM DTT 5 mM CaCl2 20% ethanol 1% CHAPS 2 M NaCl 1 mM EDTA -
Ni-IDA Resin FF Prepacked Column
$377.78 Add to cart View Product DetailsGenScript Ni-IDA Resin FF is a 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni2+ by three coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. Ni-IDA Resin FF has low Ni2+ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. Ni-IDA Resin FF Prepacked Column is an excellent choice for high performance purification of polyhistidine-tagged proteins.
Cat. No. Product Name Volume supplied Resin content L00684-12 Ni-IDA Resin FF prepacked column (1mlx2) 1mlx2 prepacked column L00684-51 Ni-IDA Resin FF prepacked column (5mlx1) 5mlx1 L00684-55 Ni-IDA Resin FF prepacked column (5mlx5) 5mlx5 Characteristics
Binding capacity >40 mg of histidine-tagged protein /ml settled resin Matrix spherical 6% highly cross-linked agarose Average particle size 90 μm (45-165 μm) Storage solution 20% ethanol Storage and Stability 18 months when stored unopened at 2-8 °C Column compatibility
The 5 mL and 1 mL columns are compatible with ÄKTA™ chromatography systems. Additional adaptors may be needed for other systems. The optimal flow rate is 1 mL/min for the 1 mL column, and 5 mL/min for the 5 mL column. For preserving the column integrity, please do not exceed a flow rate of 4 mL/min for the 1 mL column and 15 mL/min for the 5 mL column (The mobile phase of the test is aqueous buffers at room temperature and the alarm pressure is 0.3 MPa). -
Protein A Resin FF Prepacked Column
$1,796.59 Add to cart View Product DetailsGenScript Protein A Resin FF is an affinity chromatography media designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A Resin FF can also be used for immunoprecipitation of proteins, protein complexes or antigens. The recombinant protein A ligand is coupled to 4% highly cross-linked agarose. The coupling is optimized to have a high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin FF is greater than 40 mg human IgG/ ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate, etc. Table 1 lists the characteristics of Protein A Resin FF Prepacked Column.
Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly at their Fc region. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding to recombinant protein A, the Fab region is available for binding antigens.
Cat. No. Product Name Volume supplied Resin content L00680-12 Protein A Resin FF (1mlx2) 1mlx2 50% slurry L00680-51 Protein A Resin FF (5mlx1) 5mlx1 50% slurry L00680-55 Protein A Resin FF (5mlx5) 5mlx5 50% slurry Characteristics
Ligand Recombinant Streptococcal protein A expressed in E. coli Number of IgG binding sites per ligand 5 M.W. of ligand Approximately 34 kDa PI of ligand 5.17 Degree of substitution Approximately 5 mg protein A / ml settled resin Static binding capacity > 40 mg human IgG/ ml settled resin Matrix component Agarose, 4% highly cross-linked Average particle size 90 μm (45-165 μm) Storage solution 20% ethanol Storage conditions 2-8 °C Shelf life 18 months when stored unopened Column compatibility
The 5 mL and 1 mL columns are compatible with ÄKTA™ chromatography systems. Additional adaptors may be needed for other systems. The optimal flow rate is 1 mL/min for the 1 mL column, and 5 mL/min for the 5 mL column. For preserving the column integrity, please do not exceed a flow rate of 4 mL/min for the 1 mL column and 15 mL/min for the 5 mL column (The mobile phase of the test is aqueous buffers at room temperature and the alarm pressure is 0.3 MPa). -
Protein G Resin FF Prepacked Column
$2,941.13 Add to cart View Product DetailsGenScript Protein G Resin FF is an affinity chromatography medium designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. The recombinant protein G ligand is coupled to 4% highly cross-linked agarose. The static binding capacity of Protein G Resin FF is greater than 20 mg human IgG/ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate etc. Table 1 lists the characteristics of Protein G Resin FF Prepacked Column.
Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native protein G has 3 IgG binding domains and also sites for albumin and cell-surface binding. The latter have been eliminated from recombinant protein G to reduce nonspecific binding. Although protein G has very similar tertiary structures to protein A, their amino acid compositions differ significantly, resulting in different binding characteristics. Protein G can be used for purification of mammalian monoclonal and polyclonal IgGs that do not bind well to protein A. Protein G has greater affinity than protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike protein A, protein G does not bind to human IgM, IgD and IgA.
Cat. No. Product Name Volume supplied Resin content L00681-12 Protein G Resin FF (1mlx2) 1mlx2 50% slurry L00681-51 Protein G Resin FF (5mlx1) 5mlx1 50% slurry L00681-55 Protein G Resin FF (5mlx5) 5mlx5 50% slurry Storage
Store in 20% ethanol at 2°C to 8°C. Do not freeze.
CharacteristicsLigand Recombinant Streptococcal protein G lacking the albumin-binding sites expressed in E. coli Number of IgG binding sites per ligand 3 M.W. of ligand Approximately 22 kDa PI of ligand 4.69 Degree of substitution Approximately 2 mg protein G/ml settled resin Static binding capacity ≥ 20 mg human IgG/ml settled resin Matrix spherical 4% highly cross-linked agarose Average particle size 90 μm (45-165 μm) Recommended flow rate 50-300cm/h Storage solution 20% ethanol Storage and Stability 18 months when stored unopened at 2-8 °C Column compatibility
The 5 mL and 1 mL columns are compatible with ÄKTA™ chromatography systems. Additional adaptors may be needed for other systems. The optimal flow rate is 1 mL/min for the 1 mL column, and 5 mL/min for the 5 mL column. For preserving the column integrity, please do not exceed a flow rate of 4 mL/min for the 1 mL column and 15 mL/min for the 5 mL column (The mobile phase of the test is aqueous buffers at room temperature and the alarm pressure is 0.3 MPa).
