6 x 1 L

1-CHLOROBUTANE

1-METHYL-2-PYRROLIDINONE

1-METHYL-2-PYRROLIDINONE

1,2,4-TRICHLOROBENZENE

1,2,4-TRICHLOROBENZENE

1,4-Butanediol

Alcohol

Alcohol

CHLOROBENZENE

CHLOROFORM With Amylene

Chloroform with Ethanol

CYCLOHEXANE

Decahydronaphthalene, Certified Grade

Ether with Ethanol

Ether, anhydrous

ETHER, ANHYDROUS

Ethyl Acetate

ETHYLENE GLYCOL DIMETHYL ETHER

ETHYLENE GLYCOL MONOMETHYL ETHER

• • Supports growth of suspension CHO cultures to densities of over 2 x 107 cells per mL, with >95% cell viability

n

• • Uses a transfection-compatible, serum-free suspension culture medium that enables high transfection efficiencies of >90% with ExpiFectamine™ CHO transfection reagent

n

• • Supports transient transfection of suspension CHO cells at a density of 6 x 106 cells per mL

n

• • Facilitates sustained, high-level expression of high-density transiently transfected cultures, achieving yields of up to 3 g per L of recombinant protein

n

• • Supports growth and transfection of CHO cells in culture formats from <1 mL in multi-well plates to >10 L in benchtop bioreactors

n

• • Does not contain phenol red

n

Expi293™ Expression Medium is a chemically defined, serum-free, protein-free medium for growth and transfection of suspension-adapted human embryonic kidney (HEK) 293 cells. It is specifically designed as a core component to support high density culture of Expi293F™ cells in the Expi293™ Expression System for scalable transient protein expression.

• • Supports growth of suspension 293 cultures to densities of over 1.5 x 10^7 cells/mL

n

• • Transfection compatible serum-free suspension culture medium enables transfection efficiencies of greater than 70% using ExpiFectamine™ transfection reagent

n

• • Enables sustained, high level expression of high density transiently transfected cultures, achieving yields of up to 1 gram per liter of recombinant protein

n

• • Supports growth and transfection of 293 cells in culture formats of less than 1 mL in multiwell plates to greater than 10 L in disposable bioreactors

n

• • Does not contain phenol red

n

Glycerol

Glycerol

ISOBUTYL ALCOHOL

ISOPROPYL ALCOHOL

ISOPROPYL ALCOHOL

Gibco LV-MAX Production Medium is a chemically defined, serum-free, protein-free medium, specially developed for high-density growth and transfection of Gibco Viral Production Cells (suspension-adapted HEK 293-derived cells) promoting high-titer lentiviral production.

• • Supports growth of suspension Viral Production Cells to densities of over 1.0 x 10⁷ cells/mL

n

• • Enables sustained, high-level production of high-density transiently transfected cultures, achieving titer of up to 1 x 10⁸ TU/mL (unconcentrated LVVs-GFP)

n

• • Supports growth and transfection of Viral Production Cells in culture formats from 96-deep well blocks to 2-L flasks and bioreactors

n

Methyl Alcohol

Methyl Alcohol, Anhydrous Grade

N-Decane

Pentane

PROPYLENE GLYCOL

Propylene glycol monomethyl ether

Pyridine, HPLC/Spectro Grade

tert-BUTYL ALCOHOL

tert-BUTYL ALCOHOL

TETRAHYDROFURAN

Tetrahydrofuran

TETRAHYDROFURAN

Tetrahydrofuran

TOLUENE

TOLUENE

Toluene, HPLC/Spectro Grade

TRICHLOROETHYLENE

TRIETHYLAMINE

Triton® X-405, Certified Grade