Ambient

GenScript’s AmMag Block
enables protein and antibody purification from cultures up to 1 L in volume
using the AmMag SA. Magnetic beads are directly incubated in cultures or
lysates to bind the target proteins or antibodies. Once binding is complete,
the AmMag Block is used to collect the beads at the bottom of the culture
vessel, and the supernatant is discarded. The magnetic beads thus collected are
re-suspended in a smaller volume and transferred into 50 ml tubes, to be washed
and eluted automatically using the AmMag SA purification system, or manually
using the AmMag MR magnetic racks.

GenScript AmMag^TM MR magnetic separation rack enables mid-scale separation of magnetic beads. The rack is designed to separate GenScript’s paramagnetic beads from solutions contained in 15 mL or 50 mL tubes.

GenScript AmMag^TM MR-mini magnetic separation rack enables small-scale separation of magnetic beads. The rack is designed to separate GenScript’s paramagnetic beads from solutions contained in 1.5 mL and 2 mL micro-centrifuge tubes. The magnetic field has been optimized for separation of tubes < 2 mL in volume.

GenScript’s AmMag™ Ni magnetic beads are designed for purification and screening of histidine-tagged (his-tagged) proteins. The beads are highly EDTA and DTT resistant (Table2), enabling them to bind proteins in the presence of chelators. The beads are also optimized for easy cleanup and regeneration, for reuse with multiple samples. Table 1 describes the basic properties of the AmMag™ Ni magnetic beads.

GenScript’s AmMag™ Ni magnetic beads are designed for purification and screening of histidine-tagged (his-tagged) proteins. The beads are highly EDTA and DTT resistant (Table2), enabling them to bind proteins in the presence of chelators. The beads are also optimized for easy cleanup and regeneration, for reuse with multiple samples. Table 1 describes the basic properties of the AmMag™ Ni magnetic beads.

GenScript’s AmMag™ Ni magnetic beads are designed for purification and screening of histidine-tagged (his-tagged) proteins. The beads are highly EDTA and DTT resistant (Table2), enabling them to bind proteins in the presence of chelators. The beads are also optimized for easy cleanup and regeneration, for reuse with multiple samples. Table 1 describes the basic properties of the AmMag™ Ni magnetic beads.

Intended Use
AmMag™ Ultra AT protein A magbeads
are developed and optimized
for antibody purification for high throughput and high volume applications.

Principle
The sample containing
antibody is added to the AmMag™ Ultra AT protein A
magbeads and incubated on a rotor overnight or 2 hours (depending on the
concentration of samples) for the antibody to bind to the beads. The antibody
bound to the beads can be eluted off by using acidic elution facilitated by a
magnetic separation device. Magnetic separation eliminates the need for
centrifugation, minimizes the loss of sample and makes the process more user
friendly.

Material Supplied
GenScript AmMag™ Ultra AT protein A magbeads are super paramagnetic
beads with diameter of 45-100 μm, covalently coated with Ultra alkaline
tolerant protein A, After treatment with 1M NaOH for 24h, the load
decreased by no more than 10%. The beads are supplied as 25% slurry in 20% ethanol.
The AmMag™ Ultra AT protein A
magbeads have a binding capacity of 40mg human IgG per 1 ml settled beads. They
can be reused for up to 100 cycles. After 100 cycles, there was almost no decrease in
binding load.
Protein A, a bacterial cell
wall protein isolated from Staphylococcus aureus, binds to mammalian IgG’s,
mainly through the Fc regions. The alkaline tolerant protein A not only keeps
the specific binding capacity to Fc region of immunoglobulin molecules, but
also tolerates alkaline conditions and can withstand rigorous cleaning
procedures.

Intended Use
AmMag™ Ultra AT protein A magbeads
are developed and optimized
for antibody purification for high throughput and high volume applications.

Principle
The sample containing
antibody is added to the AmMag™ Ultra AT protein A
magbeads and incubated on a rotor overnight or 2 hours (depending on the
concentration of samples) for the antibody to bind to the beads. The antibody
bound to the beads can be eluted off by using acidic elution facilitated by a
magnetic separation device. Magnetic separation eliminates the need for
centrifugation, minimizes the loss of sample and makes the process more user
friendly.

Material Supplied
GenScript AmMag™ Ultra AT protein A magbeads are super paramagnetic
beads with diameter of 45-100 μm, covalently coated with Ultra alkaline
tolerant protein A, After treatment with 1M NaOH for 24h, the load
decreased by no more than 10%. The beads are supplied as 25% slurry in 20% ethanol.
The AmMag™ Ultra AT protein A
magbeads have a binding capacity of 40mg human IgG per 1 ml settled beads. They
can be reused for up to 100 cycles. After 100 cycles, there was almost no decrease in
binding load.
Protein A, a bacterial cell
wall protein isolated from Staphylococcus aureus, binds to mammalian IgG’s,
mainly through the Fc regions. The alkaline tolerant protein A not only keeps
the specific binding capacity to Fc region of immunoglobulin molecules, but
also tolerates alkaline conditions and can withstand rigorous cleaning
procedures.

Intended Use
AmMag™ Ultra AT protein A magbeads
are developed and optimized
for antibody purification for high throughput and high volume applications.

Principle
The sample containing
antibody is added to the AmMag™ Ultra AT protein A
magbeads and incubated on a rotor overnight or 2 hours (depending on the
concentration of samples) for the antibody to bind to the beads. The antibody
bound to the beads can be eluted off by using acidic elution facilitated by a
magnetic separation device. Magnetic separation eliminates the need for
centrifugation, minimizes the loss of sample and makes the process more user
friendly.

Material Supplied
GenScript AmMag™ Ultra AT protein A magbeads are super paramagnetic
beads with diameter of 45-100 μm, covalently coated with Ultra alkaline
tolerant protein A, After treatment with 1M NaOH for 24h, the load
decreased by no more than 10%. The beads are supplied as 25% slurry in 20% ethanol.
The AmMag™ Ultra AT protein A
magbeads have a binding capacity of 40mg human IgG per 1 ml settled beads. They
can be reused for up to 100 cycles. After 100 cycles, there was almost no decrease in
binding load.
Protein A, a bacterial cell
wall protein isolated from Staphylococcus aureus, binds to mammalian IgG’s,
mainly through the Fc regions. The alkaline tolerant protein A not only keeps
the specific binding capacity to Fc region of immunoglobulin molecules, but
also tolerates alkaline conditions and can withstand rigorous cleaning
procedures.

Intended Use
AmMag™ Ultra AT protein A magbeads
are developed and optimized
for antibody purification for high throughput and high volume applications.

Principle
The sample containing
antibody is added to the AmMag™ Ultra AT protein A
magbeads and incubated on a rotor overnight or 2 hours (depending on the
concentration of samples) for the antibody to bind to the beads. The antibody
bound to the beads can be eluted off by using acidic elution facilitated by a
magnetic separation device. Magnetic separation eliminates the need for
centrifugation, minimizes the loss of sample and makes the process more user
friendly.

Material Supplied
GenScript AmMag™ Ultra AT protein A magbeads are super paramagnetic
beads with diameter of 45-100 μm, covalently coated with Ultra alkaline
tolerant protein A, After treatment with 1M NaOH for 24h, the load
decreased by no more than 10%. The beads are supplied as 25% slurry in 20% ethanol.
The AmMag™ Ultra AT protein A
magbeads have a binding capacity of 40mg human IgG per 1 ml settled beads. They
can be reused for up to 100 cycles. After 100 cycles, there was almost no decrease in
binding load.
Protein A, a bacterial cell
wall protein isolated from Staphylococcus aureus, binds to mammalian IgG’s,
mainly through the Fc regions. The alkaline tolerant protein A not only keeps
the specific binding capacity to Fc region of immunoglobulin molecules, but
also tolerates alkaline conditions and can withstand rigorous cleaning
procedures.

GenScript’s AmMag Wand enables protein and antibody purification from cultures up to 2 L in volume using the AmMag SA. Magnetic beads are directly incubated in cultures or lysates to bind target proteins and antibodies. Once binding is complete, the AmMag Wand is used to collect the beads and transfer them into 50 ml tubes, to be washed and eluted automatically using the AmMag SA purification system, or manually using the AmMag MR magnetic racks.

GenScript’s AmMag Wand enables protein and antibody purification from cultures up to 2 L in volume using the AmMag SA. Magnetic beads are directly incubated in cultures or lysates to bind target proteins and antibodies. Once binding is complete, the AmMag Wand is used to collect the beads and transfer them into 50 ml tubes, to be washed and eluted automatically using the AmMag SA purification system, or manually using the AmMag MR magnetic racks.

GenScript’s AmMag Wand enables protein and antibody purification from cultures up to 2 L in volume using the AmMag SA. Magnetic beads are directly incubated in cultures or lysates to bind target proteins and antibodies. Once binding is complete, the AmMag Wand is used to collect the beads and transfer them into 50 ml tubes, to be washed and eluted automatically using the AmMag SA purification system, or manually using the AmMag MR magnetic racks.

GenScript’s AmMag Wand
enables protein and antibody purification from cultures up to 2 L in volume
using the AmMag SA. Magnetic beads are directly incubated in cultures or
lysates to bind target proteins and antibodies. Once binding is complete, the
AmMag Wand is used to collect the beads and transfer them into 50 ml tubes, to
be washed and eluted automatically using the AmMag SA purification system, or
manually using the AmMag MR magnetic racks.

Ammelide

Ammelide

Ammeline

Ammeline