Ambient

Hepatocyte Growth Factor (HGF),also known as hepatopoietin-A and scatter factor, is a pleiotropic mitogen belonging to the peptidase S1 family (plasminogen subfamily). It is produced by mesenchymal cells and acts on epithelial cells, endothelial cells and haemopoietic progenitor cells. HGF binds to the proto-oncogenic c-Met receptor to activate a tyrosine kinase signaling cascade. It regulates cell growth, motility and morphogenesis, thus it plays a pivotal role in angiogenesis, tumorogenesis and tissue regeneration.

Hepatocyte Growth Factor (HGF),also known as hepatopoietin-A and scatter factor, is a pleiotropic mitogen belonging to the peptidase S1 family (plasminogen subfamily). It is produced by mesenchymal cells and acts on epithelial cells, endothelial cells and haemopoietic progenitor cells. HGF binds to the proto-oncogenic c-Met receptor to activate a tyrosine kinase signaling cascade. It regulates cell growth, motility and morphogenesis, thus it plays a pivotal role in angiogenesis, tumorogenesis and tissue regeneration.

Hepatocyte Growth Factor (HGF),also known as hepatopoietin-A and scatter factor, is a pleiotropic mitogen belonging to the peptidase S1 family (plasminogen subfamily). It is produced by mesenchymal cells and acts on epithelial cells, endothelial cells and haemopoietic progenitor cells. HGF binds to the proto-oncogenic c-Met receptor to activate a tyrosine kinase signaling cascade. It regulates cell growth, motility and morphogenesis, thus it plays a pivotal role in angiogenesis, tumorogenesis and tissue regeneration.

c-Met, also called tyrosine-protein kinase Met or hepatocyte growth factor receptor (HGFR), is a protein that in humans is encoded by the MET gene. The protein possesses tyrosine kinase activity. The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling.

Molecular Formula : C78H123N23O22S2 • x(C2HF3O2)

Molecular Formula : C78H123N23O22S2 • x(C2HF3O2)

Molecular Formula : C78H123N23O22S2 • x(C2HF3O2)

Molecular Formula : C30H36O16

Molecular Formula : C30H36O16

Molecular Formula : C16H18BrNO3

Molecular Formula : C16H18BrNO3

Molecular Formula : C16 H17 N O3 . Cl H

Molecular Formula : C16 H17 N O3 . Cl H

Molecular Formula : C16 H17 N O3 . Cl H

GenScript
High Affinity Ni-Charged Resin is an 4% cross-linked agarose medium covalently
coupled to a chelating agent that binds Ni2+ by four coordination
sites for high-affinity purification of polyhistidine-tagged recombinant
proteins. High Affinity Ni-Charged Resin has
low Ni²⁺ leakage, high protein-binding capacity and stability, and
is compatible with a wide range of additives used in protein purification. This
makes High Affinity Ni-Charged Resin the excellent choice for high performance
purification of polyhistidine-tagged proteins. 

GenScript
High Affinity Ni-Charged Resin is an 4% cross-linked agarose medium covalently
coupled to a chelating agent that binds Ni2+ by four coordination
sites for high-affinity purification of polyhistidine-tagged recombinant
proteins. High Affinity Ni-Charged Resin has
low Ni²⁺ leakage, high protein-binding capacity and stability, and
is compatible with a wide range of additives used in protein purification. This
makes High Affinity Ni-Charged Resin the excellent choice for high performance
purification of polyhistidine-tagged proteins. 

GenScript
High Affinity Ni-Charged Resin is an 4% cross-linked agarose medium covalently
coupled to a chelating agent that binds Ni2+ by four coordination
sites for high-affinity purification of polyhistidine-tagged recombinant
proteins. High Affinity Ni-Charged Resin has
low Ni²⁺ leakage, high protein-binding capacity and stability, and
is compatible with a wide range of additives used in protein purification. This
makes High Affinity Ni-Charged Resin the excellent choice for high performance
purification of polyhistidine-tagged proteins. 

GenScript High Affinity Ni-Charged Resin FF is an 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni²⁺ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin FF has low Ni²⁺ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin FF Prepacked Column the excellent choice for high performance purification of polyhistidine-tagged proteins.

Storage
Store in 20% ethanol at 2°C to 8°C. Do not freeze.

Characteristics

Column compatibility

The 5 mL and 1 mL columns are compatible with ÄKTA™ chromatography systems. Additional adaptors may be needed for other systems. The optimal flow rate is 1 mL/min for the 1 mL column, and 5 mL/min for the 5 mL column. For preserving the column integrity, please do not exceed a flow rate of 4 mL/min for the 1 mL column and 15 mL/min for the 5 mL column (The mobile phase of the test is aqueous buffers at room temperature and the alarm pressure is 0.3 MPa).

Reagents Compatible

GenScript High Affinity Ni-Charged Resin FF is an 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni²⁺ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin FF has low Ni²⁺ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin FF Prepacked Column the excellent choice for high performance purification of polyhistidine-tagged proteins.

Storage
Store in 20% ethanol at 2°C to 8°C. Do not freeze.

Characteristics

Column compatibility

The 5 mL and 1 mL columns are compatible with ÄKTA™ chromatography systems. Additional adaptors may be needed for other systems. The optimal flow rate is 1 mL/min for the 1 mL column, and 5 mL/min for the 5 mL column. For preserving the column integrity, please do not exceed a flow rate of 4 mL/min for the 1 mL column and 15 mL/min for the 5 mL column (The mobile phase of the test is aqueous buffers at room temperature and the alarm pressure is 0.3 MPa).

Reagents Compatible

GenScript High Affinity Ni-Charged Resin FF is an 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni²⁺ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin FF has low Ni²⁺ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin FF Prepacked Column the excellent choice for high performance purification of polyhistidine-tagged proteins.

Storage
Store in 20% ethanol at 2°C to 8°C. Do not freeze.

Characteristics

Column compatibility

The 5 mL and 1 mL columns are compatible with ÄKTA™ chromatography systems. Additional adaptors may be needed for other systems. The optimal flow rate is 1 mL/min for the 1 mL column, and 5 mL/min for the 5 mL column. For preserving the column integrity, please do not exceed a flow rate of 4 mL/min for the 1 mL column and 15 mL/min for the 5 mL column (The mobile phase of the test is aqueous buffers at room temperature and the alarm pressure is 0.3 MPa).

Reagents Compatible

High Unsaturated Fat Diet

High Unsaturated Fat Diet

High Unsaturated Fat Diet

Molecular Formula : C9 H9 N O3

Molecular Formula : C9 H9 N O3

Molecular Formula : C9 H9 N O3

Molecular Formula : C9H8NNaO3

Molecular Formula : C9H8NNaO3

Molecular Formula : C9H8NNaO3

Molecular Formula : C313C6H9NO3

Molecular Formula : C313C6H9NO3

Molecular Formula : C9 2H5 H4 N O3

Molecular Formula : C9 2H5 H4 N O3

Hippuric Acid, Reagent

Hippuric Acid, Reagent

HIPPURYL-L-HISTIDYL-L-LEUCINE

Adapter for FastPrep-24 instrument. Holds 48 x 2 mL tubes for room temperature cell grinding and lysis

Molecular Formula : C9H18O2

Molecular Formula : C18 H17 O7 . Cl

Molecular Formula : C18 H17 O7 . Cl

Description

His Tag Antibody Plate is a microtiter plate coated with THE^TM Anti His Tag Antibody. The plate is designed for capturing His tagged protein with His tag located at N-terminus, C-terminus, or internal of the protein. It can be applied for analysis of different length of His tag including Hexa-His (6 His), Penta-His (5-His) and Tetra-His (4-His).The plate demonstrate high sensitivity and high binding capacity. The capacity and sensitivity varies, depending on protein size, structure and solution buffer. Generally, proteins with lower molecular weight (M.W) can be more sensitive to the His antibody plate than ones with higher M.W. Compared with Nickel Coated Plate, the affinity plate can bind target proteins with high specificity, and tolerable with several common used reagents such as EDTA, imidazole and β-ME.

Description

His Tag Antibody Plate is a microtiter plate coated with THE^TM Anti His Tag Antibody. The plate is designed for capturing His tagged protein with His tag located at N-terminus, C-terminus, or internal of the protein. It can be applied for analysis of different length of His tag including Hexa-His (6 His), Penta-His (5-His) and Tetra-His (4-His).The plate demonstrate high sensitivity and high binding capacity. The capacity and sensitivity varies, depending on protein size, structure and solution buffer. Generally, proteins with lower molecular weight (M.W) can be more sensitive to the His antibody plate than ones with higher M.W. Compared with Nickel Coated Plate, the affinity plate can bind target proteins with high specificity, and tolerable with several common used reagents such as EDTA, imidazole and β-ME.

Description

His Tag Antibody Plate is a microtiter plate coated with THE^TM Anti His Tag Antibody in a non-removable format. The plate is designed for capturing His tagged protein with His tag located at N-terminus, C-terminus, or internal of the protein. It can be applied for analysis of different length of His tag including Hexa-His (6 His), Penta-His (5-His) and Tetra-His (4-His).The plate demonstrate high sensitivity and high binding capacity. The capacity and sensitivity varies, depending on protein size, structure and solution buffer. Generally, proteins with lower molecular weight (M.W) can be more sensitive to the His antibody plate than ones with higher M.W. Compared with Nickel Coated Plate, the affinity plate can bind target proteins with high specificity, and tolerable with several common used reagents such as EDTA, imidazole and 2-ME.

GenScript His Tag ELISA Detection Kit, a 1.5 hour competition ELISA, is developed for rapid and high throughput detection of His-tagged
proteins. There are several potential usages:

1. Quickly identify the presence of His-tagged proteins in samples.
2. Optimize protein expression by monitoring the His-tagged proteins level.
3. High throughput screening of stable cell lines expressing His-tagged proteins.

This kit is based on competitive ELISA method. His Tag Plate in the kit is a 96-well microtiter
plate coated with a His-tagged protein with the molecular weight of 12.7 kDa. The
plate, which comprises of 8 wells x 12 strips, is demountable. When Anti-His Monoclonal
Antibody and His-tagged proteins are added to the well , the coated His-tagged
proteins compete with His Tag Standard (His-tagged protein with the molecular weight of 11.3
kDa) in solution or His-tagged
protein in sample to interact with Anti-His Monoclonal Antibody. The higher the
concentration of the His-tagged protein in solution is, the less the antibody
bound to the plate will be. The Anti-His Monoclonal Antibody used in the kit is
a mouse anti His tag monoclonal antibody (GenScript A00186). Antibody Tracer, which is a horseradish peroxidase (HRP) conjugated Goat anti mouse IgG, is used for enzyme reaction. His-tagged protein, Anti-His Monoclonal Antibody and antibody tracer form a complex. Other unbound molecules can be removed by washing
solution. The antibody tracer reacts with TMB substrate to develop blue
product that turns yellow immediately when the Stop Solution is added, which
can be measured by microplate reader at 450 nM.

In optimized
test condition, each absorbance value is indicated to the individual His-tagged
proteins amount in solution.  His-tagged
protein standards of known concentration and the corresponding absorbance
values are used to construct a standard curve. With the standard curve, His-tagged
protein amount present in the unknown sample can be calculated by transforming
its absorbance value. 

GenScript His-tag antibody [Biotin], pAb, Rabbit specifically reacts with fusion proteins containing His tagged fusion proteins.

GenScript His-tag Antibody, pAb, Rabbit specifically reacts with fusion proteins containing His epitope tags.For N-terminal His-tagged protein detection, THE™ Anti-His mAb (Cat.No.A00186) is recommended.