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GenScript Protein A MagBeads are superparamagnetic beads covalently bound with recombinant protein A for antibody purification. The beads are supplied as 25% slurry in phosphate buffered saline (PBS), pH 7.4, containing 20% ethanol. The Protein A MagBeads have a binding capacity of more than 10 mg Rabbit IgG per 1 ml settled beads (e.g. 4 ml 25% slurry).
Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs, mainly through Fc regions. Native Protein A has five IgG binding domains and many unknown-function repeated sequences. Recombinant Protein A only contains five high-affinity IgG binding domains to reduce nonspecific binding.

Product Details

GenScript Protein A Resin is an affinity
chromatography medium designed for easy, one-step purification of classes, subclasses
and fragments of immunoglobulins from biological fluids and from cell culture
media. Protein A Resin can also be used for immunoprecipitation of
proteins, protein complexes or antigens. The recombinant protein A ligand is
coupled to 4%
agarose. The coupling is optimized to give high binding capacity for
immunoglobulins. The static binding
capacity of Protein A Resin is greater than 20 mg human IgG/ml settled resin.
The binding capacity will
vary depending on several factors such as target antibody, flow rate etc. Table
1 lists the characteristics of Protein A Resin.

Protein A, a bacterial cell wall protein isolated
from Staphylococcus
aureus,
binds to mammalian IgGs mainly through Fc regions. Native
protein A has five IgG binding domains and many other domains with unknown
functions. Recombinant protein A contains five high
affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region
is involved in binding, the Fab region is available for binding antigens.

GenScript’s Protein A Resin FF is an affinity chromatography media designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A Resin FF can also be used for immunoprecipitation of proteins, protein complexes or antigens.The recombinant protein A ligand is coupled to 4% highly cross-linked agarose. The coupling is optimized to have a high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin FF is greater than 40 mg rabbit IgG/ ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate, etc. Table 1 lists the characteristics of Protein A Resin FF.

Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly at their Fc region. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding to recombinant protein A, the Fab region is available for binding antigens.

GenScript Protein A Resin FF is an affinity chromatography media designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A Resin FF can also be used for immunoprecipitation of proteins, protein complexes or antigens. The recombinant protein A ligand is coupled to 4% highly cross-linked agarose. The coupling is optimized to have a high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin FF is greater than 40 mg human IgG/ ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate, etc. Table 1 lists the characteristics of Protein A Resin FF Prepacked Column.

Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly at their Fc region. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding to recombinant protein A, the Fab region is available for binding antigens.

Characteristics

Column compatibility

The 5 mL and 1 mL columns are compatible with ÄKTA™ chromatography systems. Additional adaptors may be needed for other systems. The optimal flow rate is 1 mL/min for the 1 mL column, and 5 mL/min for the 5 mL column. For preserving the column integrity, please do not exceed a flow rate of 4 mL/min for the 1 mL column and 15 mL/min for the 5 mL column (The mobile phase of the test is aqueous buffers at room temperature and the alarm pressure is 0.3 MPa).

GenScript Protein A Resin FF is an affinity chromatography media designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A Resin FF can also be used for immunoprecipitation of proteins, protein complexes or antigens. The recombinant protein A ligand is coupled to 4% highly cross-linked agarose. The coupling is optimized to have a high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin FF is greater than 40 mg human IgG/ ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate, etc. Table 1 lists the characteristics of Protein A Resin FF Prepacked Column.

Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly at their Fc region. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding to recombinant protein A, the Fab region is available for binding antigens.

Characteristics

Column compatibility

The 5 mL and 1 mL columns are compatible with ÄKTA™ chromatography systems. Additional adaptors may be needed for other systems. The optimal flow rate is 1 mL/min for the 1 mL column, and 5 mL/min for the 5 mL column. For preserving the column integrity, please do not exceed a flow rate of 4 mL/min for the 1 mL column and 15 mL/min for the 5 mL column (The mobile phase of the test is aqueous buffers at room temperature and the alarm pressure is 0.3 MPa).

GenScript Protein A Resin FF is an affinity chromatography media designed for easy, one-step purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media. Protein A Resin FF can also be used for immunoprecipitation of proteins, protein complexes or antigens. The recombinant protein A ligand is coupled to 4% highly cross-linked agarose. The coupling is optimized to have a high binding capacity for immunoglobulins. The static binding capacity of Protein A Resin FF is greater than 40 mg human IgG/ ml settled resin. The dynamic binding capacity will vary depending on several factors such as target antibody, flow rate, etc. Table 1 lists the characteristics of Protein A Resin FF Prepacked Column.

Protein A, a bacterial cell wall protein isolated from Staphylococcus aureus, binds to mammalian IgGs mainly at their Fc region. Native protein A has five IgG binding domains and many other domains with unknown functions. Recombinant protein A contains five high affinity IgG binding domains with other non-essential domains removed to reduce nonspecific binding. Since only the Fc region is involved in binding to recombinant protein A, the Fab region is available for binding antigens.

Characteristics

Column compatibility

The 5 mL and 1 mL columns are compatible with ÄKTA™ chromatography systems. Additional adaptors may be needed for other systems. The optimal flow rate is 1 mL/min for the 1 mL column, and 5 mL/min for the 5 mL column. For preserving the column integrity, please do not exceed a flow rate of 4 mL/min for the 1 mL column and 15 mL/min for the 5 mL column (The mobile phase of the test is aqueous buffers at room temperature and the alarm pressure is 0.3 MPa).

GenScript Protein A/G MagBeads are superparamagnetic beads covalently bound with recombinant proteins A and G for antibody purification. The beads are supplied as 25% slurry in phosphate buffered saline (PBS), pH 7.4, containing 20% ethanol. The Protein A/G MagBeads have a binding capacity of more than 10 mg Goat IgG per 1 ml settled beads.
Protein A/G is a genetically engineered protein (MW ≈43 kDa) that combines the IgG binding sites of both Protein A and Protein G. 6×His-tag was attached to its N-terminal to facilitate the purification. The secreted Protein A/G contains four Fc-binding domains from Protein A and two from Protein G, making it a more universal tool to bind and purify immunoglobulins.

Product Details

The
GenScript Protein A/G MagBeads MX are ideal for small‐scale purification of antibodies
from  plasma, serum, ascites, and cell
culture.

The
Protein A/G is a genetically engineered protein (MW≈43 kDa) that combines the
IgG binding sites of both Protein A and Protein G. It contains four Fc‐binding
domains from Protein A and two from Protein G, making it a universal tool to
bind and purify immunoglobulins (IgGs). GenScript’s Protein A/G MagBeads MX have
Protein A/G pre-coupled on the surface of superparamagnetic beads. To purify
antibodies, a sample containing the antibody of interest is incubated with the Protein
A/G MagBeads MX. The antibody binds to the Protein A/G on the beads during a
short incubation. This is followed by washing the beads to remove
non-specifically bound materials, and eluting the purified antibody. Magnetic
separation simplify purification process by eliminating the need for
centrifugation, minimizes sample loss and removes the excessive steps of a traditional
centrifugation-based purification method.

The
GenScript Protein A/G MagBeads MX are ideal for small‐scale purification of antibodies
from  plasma, serum, ascites, and cell
culture.

The
Protein A/G is a genetically engineered protein (MW≈43 kDa) that combines the
IgG binding sites of both Protein A and Protein G. It contains four Fc‐binding
domains from Protein A and two from Protein G, making it a universal tool to
bind and purify immunoglobulins (IgGs). GenScript’s Protein A/G MagBeads MX have
Protein A/G pre-coupled on the surface of superparamagnetic beads. To purify
antibodies, a sample containing the antibody of interest is incubated with the Protein
A/G MagBeads MX. The antibody binds to the Protein A/G on the beads during a
short incubation. This is followed by washing the beads to remove
non-specifically bound materials, and eluting the purified antibody. Magnetic
separation simplify purification process by eliminating the need for
centrifugation, minimizes sample loss and removes the excessive steps of a traditional
centrifugation-based purification method.

The
GenScript Protein A/G MagBeads MX are ideal for small‐scale purification of antibodies
from  plasma, serum, ascites, and cell
culture.

The
Protein A/G is a genetically engineered protein (MW≈43 kDa) that combines the
IgG binding sites of both Protein A and Protein G. It contains four Fc‐binding
domains from Protein A and two from Protein G, making it a universal tool to
bind and purify immunoglobulins (IgGs). GenScript’s Protein A/G MagBeads MX have
Protein A/G pre-coupled on the surface of superparamagnetic beads. To purify
antibodies, a sample containing the antibody of interest is incubated with the Protein
A/G MagBeads MX. The antibody binds to the Protein A/G on the beads during a
short incubation. This is followed by washing the beads to remove
non-specifically bound materials, and eluting the purified antibody. Magnetic
separation simplify purification process by eliminating the need for
centrifugation, minimizes sample loss and removes the excessive steps of a traditional
centrifugation-based purification method.

This Antibody recognizes Protein C tagged fusion proteins.

Protein G, a cell surface protein of group G streptococci, is a Type III Fc receptor that binds to the Fc region of IgG by a non-immune mechanism similar to that of protein A from Staphylococcus aureus. This product contains three IgG binding domains, but its albumin-binding region has been removed. Additionally, His-tag and cysteine have been added to N- and C-termini, respectively, to facilitate purification and conjugation. This does not affect the protein’s ability to bind to IgG.