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GenScript High Affinity Ni-Charged Resin FF is an 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni²⁺ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin FF has low Ni²⁺ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin FF Prepacked Column the excellent choice for high performance purification of polyhistidine-tagged proteins.

Storage
Store in 20% ethanol at 2°C to 8°C. Do not freeze.

Characteristics

Column compatibility

The 5 mL and 1 mL columns are compatible with ÄKTA™ chromatography systems. Additional adaptors may be needed for other systems. The optimal flow rate is 1 mL/min for the 1 mL column, and 5 mL/min for the 5 mL column. For preserving the column integrity, please do not exceed a flow rate of 4 mL/min for the 1 mL column and 15 mL/min for the 5 mL column (The mobile phase of the test is aqueous buffers at room temperature and the alarm pressure is 0.3 MPa).

Reagents Compatible

GenScript High Affinity Ni-Charged Resin FF is an 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni²⁺ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin FF has low Ni²⁺ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin FF Prepacked Column the excellent choice for high performance purification of polyhistidine-tagged proteins.

Storage
Store in 20% ethanol at 2°C to 8°C. Do not freeze.

Characteristics

Column compatibility

The 5 mL and 1 mL columns are compatible with ÄKTA™ chromatography systems. Additional adaptors may be needed for other systems. The optimal flow rate is 1 mL/min for the 1 mL column, and 5 mL/min for the 5 mL column. For preserving the column integrity, please do not exceed a flow rate of 4 mL/min for the 1 mL column and 15 mL/min for the 5 mL column (The mobile phase of the test is aqueous buffers at room temperature and the alarm pressure is 0.3 MPa).

Reagents Compatible

GenScript High Affinity Ni-Charged Resin FF is an 6% highly cross-linked agarose medium covalently coupled to a chelating agent that binds Ni²⁺ by four coordination sites for high-affinity purification of polyhistidine-tagged recombinant proteins. High Affinity Ni-Charged Resin FF has low Ni²⁺ leakage, high protein-binding capacity and stability, and is compatible with a wide range of additives used in protein purification. This makes High Affinity Ni-Charged Resin FF Prepacked Column the excellent choice for high performance purification of polyhistidine-tagged proteins.

Storage
Store in 20% ethanol at 2°C to 8°C. Do not freeze.

Characteristics

Column compatibility

The 5 mL and 1 mL columns are compatible with ÄKTA™ chromatography systems. Additional adaptors may be needed for other systems. The optimal flow rate is 1 mL/min for the 1 mL column, and 5 mL/min for the 5 mL column. For preserving the column integrity, please do not exceed a flow rate of 4 mL/min for the 1 mL column and 15 mL/min for the 5 mL column (The mobile phase of the test is aqueous buffers at room temperature and the alarm pressure is 0.3 MPa).

Reagents Compatible

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High Capacity Cell Culture Package, includes 487-10106, 487-10142 and 487-10145

High capacity magnetic stirrer, 150L with ceramic top plate, 120V

High capacity magnetic stirrer, 50L with ceramic top plate, 120V

High Capacity Micro Rotor, Aluminum, 148 x 1.5/2.0 mL

High Capacity Micro Rotor, PTFE, 148 x 1.5/2.0 mL

High Gloss Thermal Paper for Mitsubishi P-95DW Thermal Printer (Cat. No. 731-74006)

High Performance 23 cu. ft. FMS Upright Refrigerator, +5C, 115V, 60 Hz

Molecular/Enzyme Products

Molecular/Enzyme Products

High Speed 0.2 to 0.4 mL Adapter for 2.0 mL Rotors, 6/pk

High speed 0.5 mL Adapter for 2.0 mL Rotors, 6/pk

High speed 1.5 mL Adapter for 2.0 mL Rotors, 6/pk

High Speed Centrifuge, Rotor for 1.5 and 2.0 mL Centrifuge Tubes, 500 to 12,000 rpm, 8800g

High Speed Microcentrifuge, 120V-240V, 50/60Hz, US, UK, EU Plugs (Includes: 12-Place Rotor for 1.5mL and 2.0mL Microcentrifuge Tubes)

High temperature cap (Red-PBT plastic, up to 180 degree C) GL45

High Temperature PBT Screw Caps for Storage Bottles, Open Top, GL25

High Temperature PBT Screw Caps for Storage Bottles, Open Top, GL45

High Vacuum Handheld Digital Gauge

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High Wall Tray – Large

High Wall Tray – Small

High-Capacity Sample Transport Tote (Duraporter(R) XL), Blue

High-Capacity Sample Transport Tote (Duraporter(R) XL), Red

High-Capacity Sample Transport Tote (Duraporter(R) XL), Yellow

High-Temperature Burner, Venturi Tube, Meker Top, Cylinder Gas

High-Temperature Burner, Venturi Tube, Meker Top, Natural Gas

HIGHConic III Rotor for use with Heraeus Megafuge 8 Centrifuge