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Trypan Blue

Trypan Blue

Trypan Blue

Trypan Blue is a blue acid dye with a strong affinity for cellulose. Trypan blue solution may be used in trypan blue based cytotoxitiy and proliferation assays. Trypan blue is also recommended for use in dye exclusion procedures for viable cell counting. Non-viable cells will up-take trypan blue at a faster rate than viable cells.

Trypan Blue, 100 mL

Cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts. Used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells.

• • Gentle on cells

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• • Room temperature-stable for 24 months

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• • Easy to use

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• • Animal origin-free

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• • Gentle on cells, purity increases specificity due to the action of a single enzyme and reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts

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• • Can be directly substituted for trypsin in existing protocols

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• • Dilution alone inactivates TrypLE Express, avoiding the need for trypsin inhibitors, such as FBS

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n nnEnergy efficient, less hazardous, sustainable packagingnnCell Culture, Mammalian Cell Culturen

Order Info

Shipping Condition: Room Temperature

Compliance

Manufactured at a cGMP-compliant facility that is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Used for dissociating a wide range of adherent mammalian cells, including CHO, HEK 293, A529, primary human keratinocytes, and embryonic stem cells. TrypLE™ Express cleaves peptide bonds on the C-terminal sides of lysine and arginine, and is a direct replacement for trypsin. Exceptional purity increases specificity and reduces damage to cells that can be caused by other enzymes present in some trypsin extracts.

• • Gentle On Cells

n

• • urity increases specificity due to the action of single enzyme, reducing damage caused by cleavage from enzymes present in some trypsin preparations and in other protease extracts

n

• • Room-Temperature Stable for 24 months

n

• • Ready to use

n

• • Can be directly substituted for trypsin in existing protocols

n

• • Dilution alone inactivates TrypLE Express, avoiding the need for trypsin inhibitors, such as FBS

n

Energy efficient, less hazardous, sustainable packagingnnCell Culture, Mammalian Cell Culture

Trypsin 1-250, virus free

Trypsin 1-250, Virus Free