2

Molecular Formula : C31H48O7

Molecular Formula : (C2H3Cl)n

Potassium Citrate Monobasic

Molecular Formula : C4 B N4 . K

GenScript Protein A/G MagBeads are superparamagnetic beads covalently bound with recombinant proteins A and G for antibody purification. The beads are supplied as 25% slurry in phosphate buffered saline (PBS), pH 7.4, containing 20% ethanol. The Protein A/G MagBeads have a binding capacity of more than 10 mg Goat IgG per 1 ml settled beads.
Protein A/G is a genetically engineered protein (MW ≈43 kDa) that combines the IgG binding sites of both Protein A and Protein G. 6×His-tag was attached to its N-terminal to facilitate the purification. The secreted Protein A/G contains four Fc-binding domains from Protein A and two from Protein G, making it a more universal tool to bind and purify immunoglobulins.

Product Details

The
GenScript Protein A/G MagBeads MX are ideal for small‐scale purification of antibodies
from  plasma, serum, ascites, and cell
culture.

The
Protein A/G is a genetically engineered protein (MW≈43 kDa) that combines the
IgG binding sites of both Protein A and Protein G. It contains four Fc‐binding
domains from Protein A and two from Protein G, making it a universal tool to
bind and purify immunoglobulins (IgGs). GenScript’s Protein A/G MagBeads MX have
Protein A/G pre-coupled on the surface of superparamagnetic beads. To purify
antibodies, a sample containing the antibody of interest is incubated with the Protein
A/G MagBeads MX. The antibody binds to the Protein A/G on the beads during a
short incubation. This is followed by washing the beads to remove
non-specifically bound materials, and eluting the purified antibody. Magnetic
separation simplify purification process by eliminating the need for
centrifugation, minimizes sample loss and removes the excessive steps of a traditional
centrifugation-based purification method.

GenScript Protein G MagBeads are superparamagnetic beads covalently bound with recombinant protein G for antibody purification. The beads are supplied as 25% slurry in phosphate buffered saline (PBS), pH 7.4, containing 20% ethanol. The Protein G MagBeads have a binding capacity of more than 10 mg Goat IgG per 1 ml settled beads.
Protein G, a bacterial cell wall protein isolated from group G Streptococci, binds to mammalian IgGs mainly through Fc regions. Native Protein G has three IgG binding domains and also the sites for albumin and cell-surface binding. Albumin and cell-surface binding domains have been eliminated from recombinant Protein G to reduce nonspecific binding. Protein G has greater affinity than Protein A for most mammalian IgGs, especially for certain subclasses including human IgG3, mouse IgG1 and rat IgG2a. Unlike Protein A, Protein G does not bind to human IgM, IgD and IgA.

Product Details

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